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bioRxiv · 10.1101/2025.04.25.650549

Mechanical unloading coupled with coronary reperfusion reduces fibrosis and stimulates cardiomyocyte proliferation after myocardial infarction

Bello, S. O.·Singh, C.·Perbellini, F.·Punjabi, P. P.·Terracciano, C. M.
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Abstract

IntroductionPercutaneous left ventricular assist devices (pLVADs) have become essential tools during coronary reperfusion in high risk PCI. Significant reduction in infarct propagation is observed when mechanical unloading is coupled with reperfusion, but little is known of the effect this reduction in wall stress and extracellular matrix with pLVADs have on the hearts regenerative capacity. ObjectiveThis study investigates the effect coronary reperfusion coupled with mechanical unloading has on myocardial fibrosis, and the impact these changes in extracellular matrix have on the hearts regenerative potential. MethodsMI was induced by coronary artery ligation in Lewis rats. Hearts underwent permanent coronary ligation (AMI) or were reperfused after 90 minutes (AMI/R). In each group, hearts were either loaded (AMI-L or AMI/R-L) or unloaded (AMI-U or AMI/R-U). In the unloaded subgroup, the infarcted hearts were explanted after 90 minutes and transplanted into the abdomen of healthy recipients via heterotopic abdominal heart-lung transplantation. The recipients heart acted as control. Hearts were analysed on day 7. Results30 hearts were studied. In the permanent ligation group, fibrosis increased in both the loaded and unloaded hearts with no significant rise in cardiomyocyte proliferation. After coronary reperfusion, there was a decrease in fibrosis with mechanical unloading and cardiomyocyte proliferation rose significantly (AMI/R-L vs AMI/R-U p= 0.0001). Cardiomyocyte proliferative rate in the loaded and unloaded hearts was 0.6%, and 3.7% respectively after permanent ligation, and 0.5%, and 10.4% respectively after coronary reperfusion. ConclusionThese data show that coronary reperfusion coupled with mechanical unloading reduces myocardial fibrosis and upregulates cardiomyocyte proliferation after myocardial infarction. TRANSLATIONAL PERSPECTIVEThis study demonstrates that the adult hearts intrinsic regenerative capacity is significantly augmented when coronary reperfusion is coupled with mechanical unloading after MI in an animal model. This finding has significant translational implications for myocardial self- repair and recovery especially in ischaemic cardiomyopathy. The investigation of cardiomyocyte proliferative response should be included in current ongoing trials such as the PROTECT IV, RECOVER IV, and IMPACT trials, all currently investigating clinical outcomes when mechanical unloading is coupled with coronary reperfusion after MI.

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Bello, S. O., Singh, C., Perbellini, F., Punjabi, P. P., Terracciano, C. M.. 2025-04-28. Mechanical unloading coupled with coronary reperfusion reduces fibrosis and stimulates cardiomyocyte proliferation after myocardial infarction. https://doi.org/10.1101/2025.04.25.650549

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