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bioRxiv · 10.1101/2025.04.23.650263

Developmental dynamics and axonal transport of cerebral cortex projection neuron mRNAs in vivo

Veeraraghavan, P.·Tillman, D. E.·Ross, M. I.·Macklis, J. D.
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Abstract

Appropriate development of long-range nervous system circuitry requires dynamic regulation of subcellular mRNA localization, but little is known about how specific neuron subtypes control this critical process in vivo. Here, we employ an integrated genetic and temporally controlled approach to investigate in vivo developmental mRNA dynamics in somata and axons of a prototypical cerebral cortex projection neuron subtype, callosal projection neurons (CPN), which connect the cortical hemispheres to integrate sensorimotor information, and regulate high-level associative cognition and behavior. We identify cis-regulatory elements that are associated with mRNA turnover in CPN somata, including specific 3 untranslated regions (UTRs), and elucidate distinct modes of axon transport in CPN axons for function-specific mRNA classes. Together, these findings elucidate how developing CPN control subcellular mRNA localization, and how dysregulation of these processes might lead to neurodevelopmental and/or neurodegenerative disorders. More broadly, this work identifies general mRNA localization mechanisms that likely function across projection neuron subtypes, and in other polarized cells.

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Veeraraghavan, P., Tillman, D. E., Ross, M. I., Macklis, J. D.. 2025-04-23. Developmental dynamics and axonal transport of cerebral cortex projection neuron mRNAs in vivo. https://doi.org/10.1101/2025.04.23.650263

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