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bioRxiv · 10.1101/2025.04.18.649305

Non-DNA-damaging DNA-PK activation improving hearing and prolonging life due to NAD+ and SIRT upregulation

Abstract

Emerging evidence strongly supports a close relationship between age-related hearing loss and frailty, highlighting the importance of early detection and intervention. Recently, we invented a mitochondria-homing drug named mitochonic acid 5 (MA-5), that increases the adenosine triphosphate (ATP) levels, rescue mitochondrial function, and protect tissue damages. Currently, the phase I clinical trial has been finished in Japan (jRCT2031210495) and the phase 2 clinical trial has already been approved by PMDA. Here we show that MA-5 improved various types of hearing loss in mouse models. Structural chemical bioanalysis revealed that MA-5 is a mixture of equal amount of S- and R- enantiomer and both S- and R- enantiomer increase ATP by binding mitochondrial protein, mitofilin. However, S-enantiomer significantly increased the NAD+ levels by binding to the NAD+-producing key enzyme nicotinamide phosphoribosyltransferase (NAMPT). Moreover, the S-enantiomer increased the sirtuin 1 protein by suppressing polyubiquitination induced by tripartite motif containing 28 (TRIM28) phosphorylation which was triggered by DNA-dependent protein kinase (DNA-PK) activation in the absence of DNA damage. Transcriptomic signatures showed that the signature of MA-5 shows an inverse correlation with aging and mortality and is oriented in the same direction as the OSKM-related iPSCs, suggesting the modification of aging pathways. Oral administration of MA-5 to mitochondrial disease model mouse showed increased survival. Our findings suggest that, in addition to enhancing ATP levels, the coordinated regulation of NAD+ metabolism, SIRT protein expression, and DNA-PK activity-constituting a novel therapeutic triad may contribute to the amelioration of hearing impairment and mitochondrial dysfunction, thereby improving life prognosis.

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BibTeXRIS

Honkura, Y., Suzuki, T., Kujirai, R., Kanno, S., Matsumoto, Y., Tanaka, Y., Kowit, H., Nagatoishi, S., Tyshkovskiy, A., Kasahara, T., Tongu, Y., Bohan, Z., Kashiwagi, H., Saegusa, C., Fujioka, M., Usami, R., Chikuma, S., Tokifuji, Y., Matsuhashi, T., Oikawa, Y., Komatsu, H., Murayama, K., Sugasawa, T., Nanto-Hara, F., Taguchi, K., Saigusa, D., Suzuki, C., Sato, T., Suzuki, J., Owada, Y., Niizuma, K., Endo, H., Hashimoto, K., Toyohara, T., Tsumoto, K., Anderson, P., Gladyshev, V. N., Hayashi, K.-i., Katori, Y., Tomioka, Y., Abe, T.. 2025-04-23. Non-DNA-damaging DNA-PK activation improving hearing and prolonging life due to NAD+ and SIRT upregulation. https://doi.org/10.1101/2025.04.18.649305

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