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bioRxiv · 10.1101/2025.04.17.647389

PyFuRNAce: An integrated design engine for RNA origami

Monari, L.·Braun, I.·Poppleton, E.·Göpfrich, K.
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Abstract

To realize the full potential of RNA nanotechnology and RNA origami, user-friendly design tools are needed. Here, we present pyFuRNAce, an open-source, Python-based software package with a graphical user interface that enables the design of complex RNA nanostructures. PyFuRNAce integrates the entire RNA origami workflow--from motif definition and blueprint design to sequence generation and primer selection--into a single, user-friendly platform. Built around a motif-based assembly paradigm, the software enables users to create and modify custom nanostructures through an intuitive web interface with streamlined design steps and real-time 3D visualization. By consolidating multiple design stages into a unified environment, pyFuRNAce reduces the entry barrier for RNA nanotechnology and accelerates the development of functional RNA origami structures for applications in medicine, biotechnology, and synthetic biology.

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Monari, L., Braun, I., Poppleton, E., Göpfrich, K.. 2025-04-18. PyFuRNAce: An integrated design engine for RNA origami. https://doi.org/10.1101/2025.04.17.647389

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