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bioRxiv · 10.1101/2025.04.07.644581

The genome sequence of the hemlock woolly adelgid, Adelges tsugae Annand 1924

Brunet, B. M. T.·Dial, D. T.·Burke, G. R.·von Dohlen, C. D.·Freitas, J. D.·Sanderson, H.·Chida, A. R.·Jones, S. J.·Martin, F. J.·Haggerty, L.·Scherer, S. W.·Ragoussis, I.·Jones, S. J. M.·Foottit, R. G.·Havill, N. P.
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Abstract

The genome of the hemlock woolly adelgid (Adelges tsugae) is presented in 10 chromosomal pseudomolecules along with 132 unplaced scaffolds for a combined length of 216.56 Mb. The genome is highly contiguous, with a benchmarking universal single-copy orthologs (BUSCO) completion score of 98.25%, an N50 of 20.54 Mb, and the longest scaffold 37.41 Mb in length. On the basis of chromosomal synteny with other aphid genomes, the hemlock woolly adelgids genome is composed of 8 autosomes and two sex (X) chromosomes. Annotation of the assembly identified 11,800 coding genes, 1,930 non-coding genes, and 20,403 mRNA transcripts. The mitogenome is also presented in a single, annotated, circular contig 25,980 bases in length. Species taxonomyEukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Adelgoidea; Adelgidae; Adelges; Annandina; Adelges (Annandina) tsugae Annand, 1924 (NCBI:txid357502)

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Brunet, B. M. T., Dial, D. T., Burke, G. R., von Dohlen, C. D., Freitas, J. D., Sanderson, H., Chida, A. R., Jones, S. J., Martin, F. J., Haggerty, L., Scherer, S. W., Ragoussis, I., Jones, S. J. M., Foottit, R. G., Havill, N. P.. 2025-04-08. The genome sequence of the hemlock woolly adelgid, Adelges tsugae Annand 1924. https://doi.org/10.1101/2025.04.07.644581

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