Light propofol anaesthesia for non-invasive auditory EEG recording in unrestrained non-human primates.

Piette, T.; Lacaux, C.; Scheltienne, M.; Sterpenich, V.; Isnardon, M.; Moulin, V.; Cermolacce, A.; Grandjean, D.; Meguerditchian, A.; Deaux, E.; Giraud, A.-L.

doi:10.1101/2025.03.24.644890

bioRxiv · 10.1101/2025.03.24.644890

Light propofol anaesthesia for non-invasive auditory EEG recording in unrestrained non-human primates.

Piette, T.·Lacaux, C.·Scheltienne, M.·Sterpenich, V.·Isnardon, M.·Moulin, V.·Cermolacce, A.·Grandjean, D.·Meguerditchian, A.·Deaux, E.·Giraud, A.-L.

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Abstract

Non-invasive electroencephalographic (EEG) experiments have been instrumental in advancing our understanding of the brain mechanisms involved in the production and perception of sounds and human speech. Performing similar experiments in non-human primates (NHPs) would help further deepen our knowledge by allowing us to investigate the evolutionary roots of these processes. However, performing EEG on NHPs is a challenge, given its sensitivity to motion artefacts, device cost and durability, and animal training requirements. For these reasons, most attempts have used invasive intracranial recordings, which led us to develop an alternative that minimises stress and prioritises animal welfare. By using mild propofol sedation, neurophysiological experimentation can easily be integrated into the routine sanitary checks of captive animals and allows the optimisation of both EEG quality and animal welfare. To assess the influence of propofol on brain activity in NHPs, we sedated three olive baboons (Papio anubis), scored their sleep stages under different doses, and recorded auditory event-related potentials (ERP)in response to grunts. Analyses of the EEG recordings with regards to sleep stage and ERP components indicate that at low dose (< 0.1mg/kg/h), propofol induces a light sleep state conducive to recording stimulus-elicited auditory activity. Overall, this experiment confirms the use of propofol sedation as an appropriate technique to study auditory processes through unrestrained, non-invasive EEG in NHPs.

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Piette, T., Lacaux, C., Scheltienne, M., Sterpenich, V., Isnardon, M., Moulin, V., Cermolacce, A., Grandjean, D., Meguerditchian, A., Deaux, E., Giraud, A.-L.. 2025-03-24. Light propofol anaesthesia for non-invasive auditory EEG recording in unrestrained non-human primates.. https://doi.org/10.1101/2025.03.24.644890

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