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bioRxiv · 10.1101/2025.03.22.643860

Promoter replacement by genome editing creates gain-of-function traits in Arabidopsis

Nobusawa, T.·Nakano, M.·Nagashima, Y.·Kusaba, M.
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Abstract

We introduced two targeted DNA double-strand breaks on the same Arabidopsis chromosome using CRISPR-Cas9 and replaced the FLOWERING LOCUS T (FT) promoter with that of a histone variant gene through chromosomal inversion. The resulting lines misexpressed FT and flowered early, like FT-overexpressing transgenic plants. This system can be used to create gain-of-function mutations that modify target gene expression as desired without incorporating foreign DNA sequences.

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Nobusawa, T., Nakano, M., Nagashima, Y., Kusaba, M.. 2025-03-25. Promoter replacement by genome editing creates gain-of-function traits in Arabidopsis. https://doi.org/10.1101/2025.03.22.643860

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