Functional and structural profiling of circulation via genetically encoded modular fluorescent probes.

Vittani, M.; Lee, A. B.; Wang, X.; Hiraoka, Y.; Konno, A.; Knak, P. G.; Kusk, P.; Nagao, M.; Asiminas, A.; Courtin, J.; Putranto, M. F.; Nasu, Y.; Tsuno, S.; Ueda, K.; Osuga, Y.; Tsuboi, T.; Bienvenu, T.; Terunuma, M.; Hirai, H.; Nedergaard, M.; Tanaka, K.; Hirase, H.

doi:10.1101/2025.03.18.643859

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bioRxiv · 10.1101/2025.03.18.643859

Functional and structural profiling of circulation via genetically encoded modular fluorescent probes.

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Abstract

Sustained labeling of fluids is crucial for their investigation in animal models. Here, we introduce a mouse line (Alb-mSc-ST), where blood and interstitial fluid are labeled with the red fluorescent protein mScarlet and SpyTag. The SpyTag-SpyCatcher technology is exploited to monitor circulating fluid properties by biosensors or detect blood-brain barrier disruption. This approach represents a valuable tool for studying vascular structure, permeability and microenvironment in body organs in vivo.

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Vittani, M., Lee, A. B., Wang, X., Hiraoka, Y., Konno, A., Knak, P. G., Kusk, P., Nagao, M., Asiminas, A., Courtin, J., Putranto, M. F., Nasu, Y., Tsuno, S., Ueda, K., Osuga, Y., Tsuboi, T., Bienvenu, T., Terunuma, M., Hirai, H., Nedergaard, M., Tanaka, K., Hirase, H.. 2025-03-18. Functional and structural profiling of circulation via genetically encoded modular fluorescent probes.. https://doi.org/10.1101/2025.03.18.643859

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