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bioRxiv · 10.1101/2025.03.14.643077

A graphical user interface for wrmXpress 2.0 streamlines helminth phenotypic screening

Caterer, Z.·Horejsi, R. V.·Weber, C.·Mathisen, B.·Nelson, C. N.·Bagatta, M.·Coughlin, I.·Wettstein, M.·Kulshrestha, A.·Lee, H. S. B.·Nunn, L. R.·Zamanian, M.·Wheeler, N. J.
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Abstract

Image-based phenotypic screening is a fundamental technique used to better understand the basic biology of helminths and advance discovery of new anthelmintics. Miniaturization of screening platforms and automated microscopy have led to a surge in imaging data and necessitated software to organize and analyze these data. Traditionally, these analyses are performed remotely on high-performance computers, often requiring an understanding of a command-line interface (CLI) and the ability to write scripts to control the software or job scheduler. Requiring access to specialized computing equipment and advanced computational skills raises the barrier to entry for these sorts of studies. The development of efficient, performant computer and graphical processing units for personal computers and cheaper imaging solutions has made the requirement of remote servers superfluous for many small to medium-scale screens, but most analytical software still require interaction with a CLI. To democratize the analysis of image-based phenotypic screens, we have developed a graphical user interface (GUI) for wrmXpress, a tool that integrates many popular computational pipelines for analyzing imaging data of parasitic and free-living worms. The GUI operates on any personal computer using the operating systems native web browser, allowing users to configure and run analyses using a point-and-click approach. Containerization of the application eliminates the need to install specialized programming libraries and dependencies, further increasing the ease of use. GUI development required a substantial reorganization of the wrmXpress backend codebase, which allowed for the addition a new pipeline for high-resolution tracking of worm behavior, and we demonstrate its functionality by showing that praziquantel modulates the behavior of Schistosoma mansoni miracidia. These advances make cutting-edge analyses of image-based phenotyping of worms more equitable and accessible.

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Caterer, Z., Horejsi, R. V., Weber, C., Mathisen, B., Nelson, C. N., Bagatta, M., Coughlin, I., Wettstein, M., Kulshrestha, A., Lee, H. S. B., Nunn, L. R., Zamanian, M., Wheeler, N. J.. 2025-03-17. A graphical user interface for wrmXpress 2.0 streamlines helminth phenotypic screening. https://doi.org/10.1101/2025.03.14.643077

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