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bioRxiv · 10.1101/2025.03.03.641235

A biphasic trajectory for maize stalk mechanics shaped by internal and external factors

Ikiriko, I. I.·Hostetler, A. N.·Reneau, J. W.·Betts, A. K.·Sparks, E. E.
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Abstract

Stalk mechanical properties impact plant stability and interactions with pathogenic microorganisms. The evaluation of stalk mechanics has focused primarily on the end-of-season outcomes and defined differences among inbred and hybrid maize genotypes. However, there is a gap in understanding how these different end-of-season outcomes are achieved. This study measured stalk flexural stiffness in maize inbred genotypes across multiple environments and in maize commercial hybrid genotypes under different disease states. Under all conditions, stalk flexural stiffness followed a biphasic trajectory, characterized by a linear increase phase and a sustained phase. Within a genotype, the environment or disease state altered the rate of increase in the linear phase but did not impact the timing of transition to the sustained phase. Whereas between genotypes, the timing of transition between phases varied. Destructive 3-point bend tests of inbred stalks showed that the trajectory of stalk mechanics is defined by the bending modulus, not the geometry. Together, these results define a biphasic trajectory of maize stalk mechanics that can be modulated by internal and external factors. This work provides a foundation for breeding programs to make informed decisions when selecting for optimized stalk mechanical trajectories, which is necessary for enhancing resilience to environmental stresses. HIGHLIGHTSO_LIA common trajectory of stalk mechanics was found across genotypes and conditions. C_LIO_LITransition between phases of stalk mechanics is inherent to genotype. C_LIO_LIThe rate of linear increase phase is modulated within a genotype by environmental or biotic factors. C_LIO_LIMaterial properties determine the trajectory of stalk mechanics. C_LI

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Ikiriko, I. I., Hostetler, A. N., Reneau, J. W., Betts, A. K., Sparks, E. E.. 2025-03-07. A biphasic trajectory for maize stalk mechanics shaped by internal and external factors. https://doi.org/10.1101/2025.03.03.641235

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