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bioRxiv · 10.1101/2025.02.28.640832

The Structural Dynamics of IRE1 and its Interaction with Unfolded Peptides

Spinetti, E.·Karagöz, G. E.·Covino, R.
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Abstract

The unfolded protein response (UPR) is a crucial signaling network that preserves endoplasmic reticulum (ER) homeostasis, impacting both health and disease. When ER stress occurs, often due to an accumulation of unfolded proteins in the ER lumen, the UPR initiates a broad cellular program to counteract cytotoxic effects. Inositol-requiring enzyme 1 (IRE1), a conserved ER-bound protein, is a key sensor of ER stress and activator of the UPR. While biochemical studies confirm IRE1s role in recognizing unfolded polypeptides, high-resolution structures showing direct interactions remain elusive. Consequently, the precise structural mechanism by which IRE1 senses unfolded proteins is debated. In this study, we employed advanced molecular modeling and 136,7 s of atomistic molecular dynamics simulations to clarify how IRE1 detects unfolded proteins. Our results demonstrate that IRE1s luminal domain directly interacts with unfolded peptides and reveal how these interactions can stabilize higher-order oligomers. We provide a detailed molecular characterization of unfolded peptide binding, identifying two distinct binding pockets at the dimers center, separate from its central groove. Furthermore, we present high-resolution structures illustrating how BiP associates with IRE1s oligomerization interface, thus preventing the formation of larger complexes. Our structural model reconciles seemingly contradictory experimental findings, offering a unified perspective on the diverse sensing models proposed. We elucidate the structural dynamics of unfolded protein sensing by IRE1, providing key insights into the initial activation of the UPR.

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Spinetti, E., Karagöz, G. E., Covino, R.. 2025-03-03. The Structural Dynamics of IRE1 and its Interaction with Unfolded Peptides. https://doi.org/10.1101/2025.02.28.640832

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