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bioRxiv · 10.1101/2025.02.28.640818

sedimix: A workflow for the analysis of hominin nuclear DNA sequences from sediments

Xu, J.·Zavala, E. I.·Moorjani, P.
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Abstract

SummarySediment DNA--the ability to extract DNA from archaeological sediments-- is an exciting new frontier in ancient DNA research, offering the potential to study individuals at a given archaeological site without destructive sampling. In recent years, several studies have demonstrated the promise of this approach by recovering hominin DNA from prehistoric sediments, including those dating back to the Middle or Late Pleistocene. However, a lack of open-source workflows for analysis of hominin sediment DNA samples poses a challenge for data processing and reproducibility of findings across studies. Here we introduce a snakemake workflow, sedimix, for processing genomic sequences from archaeological sediment DNA samples to identify hominin sequences and generate relevant summary statistics to assess the reliability of the pipeline. By performing simulations and comparing to published studies, we show that sedimix has high sensitivity and precision. sedimix offers a reliable and adaptable framework to aid in the analysis of sediment DNA datasets and improve reproducibility across studies. Availability and Implementationsedimix is available as an open-source software with the associated code and user manual available at https://github.com/jierui-cell/sedimix ContactJierui Xu (jierui.xu@berkeley.edu) Supplementary informationSupplementary data are available at Bioinformatics online

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Xu, J., Zavala, E. I., Moorjani, P.. 2025-03-02. sedimix: A workflow for the analysis of hominin nuclear DNA sequences from sediments. https://doi.org/10.1101/2025.02.28.640818

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