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bioRxiv · 10.1101/2025.02.26.640334

Aphid effector Mp10 balances immune suppression and defence activation through EDS1-dependent modulation of plant DAMP responses

Gravino, M.·Mugford, S. T.·Pontiggia, D.·Joyce, J.·Drurey, C.·Prince, D. C.·Cervone, F.·De Lorenzo, G.·Hogenhout, S. A.
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Abstract

O_LIDamage-associated molecular pattern (DAMP)-triggered immunity (DTI) serves as a crucial first line of defence against aphid attack, yet the mechanisms by which aphids suppress this response remain unclear. C_LIO_LIBy investigating the colonisation of Arabidopsis thaliana by the highly polyphagous peach-potato aphid (Myzus persicae), we identified cell wall-derived DAMPs, specifically oligogalacturonides (OGs), as key in inducing DTI against aphids. The OG-responsive immune components BAK1/BKK1, CPK5/CPK6, GRP3, and EDS1 collectively contribute to DTI limiting aphid colonisation. C_LIO_LIWe found that aphids limit OG production during feeding. Additionally, the salivary chemosensory protein (CSP) effector Mp10/CSP4, which is known to be delivered into the cytoplasm of plant cells early in aphid attack, inhibits OG-induced DTI. C_LIO_LIWhile Mp10 suppresses OG-induced DTI, it also interacts with EDS1-mediated defences, enhancing aphid fecundity in the absence of EDS1 but restoring OG responsiveness under attack, revealing its broader role in immune modulation and effector-driven host adaptation. C_LI

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Gravino, M., Mugford, S. T., Pontiggia, D., Joyce, J., Drurey, C., Prince, D. C., Cervone, F., De Lorenzo, G., Hogenhout, S. A.. 2025-02-28. Aphid effector Mp10 balances immune suppression and defence activation through EDS1-dependent modulation of plant DAMP responses. https://doi.org/10.1101/2025.02.26.640334

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