Luminos: open-source software for bidirectional microscopy

Itkis, D. G.; Brooks, F. P.; Davis, H. C.; Hotter, R.; Wong-Campos, J. D.; Qi, Y.; Jia, B. Z.; Howell, M.; Xiong, M.; Hayward, R. F.; Lee, B. H.; Wang, Y.; Perelman, R. T.; Cohen, A. E.

doi:10.1101/2025.02.22.639658

bioRxiv · 10.1101/2025.02.22.639658

Luminos: open-source software for bidirectional microscopy

Itkis, D. G.·Brooks, F. P.·Davis, H. C.·Hotter, R.·Wong-Campos, J. D.·Qi, Y.·Jia, B. Z.·Howell, M.·Xiong, M.·Hayward, R. F.·Lee, B. H.·Wang, Y.·Perelman, R. T.·Cohen, A. E.

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Abstract

Bidirectional microscopy (BDM) combines simultaneous targeted optical perturbation and imaging of biophysical or biochemical signals (e.g. membrane voltage, Ca2+, or signaling molecules). A core challenge in BDM is precise spatial and temporal alignment of stimulation, imaging, and other experimental parameters. Here we present Luminos, an open-source MATLAB library for modular and precisely synchronized control of BDM experiments. The system supports hardware-triggered synchronization across stimulation, recording, and imaging channels with microsecond accuracy. Source code and documentation for Luminos are available online at https://www.luminosmicroscopy.com and https://github.com/adamcohenlab/luminos-microscopy. This library will facilitate development of bidirectional microscopy methods across the biological sciences.

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Itkis, D. G., Brooks, F. P., Davis, H. C., Hotter, R., Wong-Campos, J. D., Qi, Y., Jia, B. Z., Howell, M., Xiong, M., Hayward, R. F., Lee, B. H., Wang, Y., Perelman, R. T., Cohen, A. E.. 2025-02-27. Luminos: open-source software for bidirectional microscopy. https://doi.org/10.1101/2025.02.22.639658

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