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bioRxiv · 10.1101/2025.02.17.638610

Betaproteobacterial clade II nosZ activated under high N2O concentrations in paddy soil microcosms

Abstract

AimsMicrobial communities in paddy soils act as potential sinks of nitrous oxide (N2O), a notorious greenhouse gas, but their potential to reduce external N2O is unclear. The direct observation of N2O reduction in submerged field soils is technically difficult. Here, we aimed to identify soil microbial clades that underpin the strong N2O mitigation capacity. Methods and ResultsWe constructed paddy soil microcosms with external N2O amendment that enabled the simultaneous evaluation of N2O reductase gene (nosZ) transcripts and N2O consumption. Although the amount of N2O amended was large, it was mostly consumed after 6- 8 days of microcosm incubation. Metatranscriptomic sequencing revealed that betaproteobacterial nosZ, especially those classified as clade II nosZ belonging to the orders Rhodocyclales or Nitrosomonadales, occupied >50% of the nosZ transcripts in three of the five paddy soils used. On the other hand, publicly available shotgun metagenomic sequences of 46 paddy soils were not dominated by betaproteobacterial clade II nosZ sequences, although they were ubiquitous. The same applied to the 16S rRNA sequences of Rhodocyclales or Nitrosomonadales. ConclusionsThe results indicated that betaproteobacterial N2O reducers potentially serve as powerful N2O sinks. Betaproteobacteria holding clade II nosZ can be targets of biostimulation, although further studies are required to understand their ecophysiology. Impact StatementOur results indicate that Rhodocyclales or Nitrosomonadales have the potential to serve as powerful N2O sinks in soils.

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BibTeXRIS

Mise, K., Masuda, Y., Senoo, K., Itoh, H.. 2025-02-19. Betaproteobacterial clade II nosZ activated under high N2O concentrations in paddy soil microcosms. https://doi.org/10.1101/2025.02.17.638610

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