bioRxiv Science⌕ Search

bioRxiv · 10.1101/2025.02.10.637374

Refining type and timing of measured crop variables for the calibration of a new winter wheat cultivar in the STICS crop model

Abstract

Crop models need to be regularly upgraded with parametrization for new cultivars but this requires calibration, which is a major challenge. With winter wheat cultivar Rubisko as a case study, we propose to apply a calibration protocol to estimate the parameters of this new cultivar with multi-trials experimental data. We tested the calibration protocol in different conditions including or not LAI and/or biomass experimental data and we found that the resulting LAI and biomass dynamics strongly diverge. Several key findings emerge from this study: (1) RUE parameters should be excluded from the calibration process, as their critical role in biomass dynamics causes the optimization algorithm to treat them as adjustment parameters, resulting in unrealistic values for multiple parameters; (2) either LAI or biomass variables alone are sufficient for calibration, enabling experimental efforts to focus on one variable rather than both; and (3) the use of a synthetic dataset has facilitated the identification of the optimal type and timing of data collection needed to parameterize a new variety in the model. Moreover, the proposed methodology offers extrapolatable solutions applicable to other contexts (e.g., different models or datasets) and provides guidance on acquiring the most effective dataset for optimal calibration. The unbalanced structure of our dataset also highlighted the need to mobilize other calibration criteria (weighted RMSE) and alternative solutions to bridge the gap between quantitative metrics and empirical visual assessments.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Gawinowski, M., Aubry, M., Buis, S., Garcia, C., Deswarte, J.-C., Bancal, M.-O., Launay, M.. 2025-02-11. Refining type and timing of measured crop variables for the calibration of a new winter wheat cultivar in the STICS crop model. https://doi.org/10.1101/2025.02.10.637374

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

Loss of starch synthase IIa alleviates the negative impact of high temperature on rice starch during grain filling

High temperatures during grain filling stage are becoming increasingly frequent, compromising both grain and eating quality and thereby driving demand for heat-resilient cultivars. Such conditions are known to reduce the expression of granule-bound starch synthase I (GBSSI) and starch branching enzyme IIb (BEIIb), which are involved in starch biosynthesis, resulting in a decrease in amylose content and an increase in long-chain amylopectin. Thus, the present study introduced functional mutation in starch synthase IIa (SSIIa) that increases the proportion of short amylopectin chains to genetically compensate for the high-temperature-induced increase in amylopectin long chain. Rice lines carrying the ss2a mutation were grown at two locations with cooler (Akita) and warmer (Okayama) temperatures. Their grain traits, starch structure, and eating quality were compared. The ss2a mutant lines showed an increased proportion of short amylopectin chains as well as an increased apparent amylose content. Furthermore, these alterations in starch structure varied with the grain-filling temperature of the cultivation sites, ultimately affected eating quality. These results suggest that enriching short amylopectin chain via the ss2a mutation can counteract the increase in long amylopectin chain caused by high temperatures during grain filling, thereby maintaining a desirable starch structure and eating quality.

plant biology↗

Analysis of SpCas9 on- and off-target effects in high efficiency multiplex editing in Arabidopsis

RNA-guided nucleases (RGNs), such as Cas9 from Streptococcus pyogenes (SpCas9), are widely used for plant genome editing. Previous surveys for off-targeting, the modification of unintended targets with similarity to the intended target, indicate high specificity of SpCas9 in plant cells. However, off-targeting has not been assessed for efficiency-optimized editing systems combined with extensive multiplexing, which might increase the likelihood of cleavage at unintended sites. We therefore analyzed Arabidopsis thaliana lines that had been extensively mutagenized using zCas9i and up to 29 gRNAs addressing >45 target sites over several rounds of editing. Genomes were sequenced by short- and long-read technologies, and genome-wide variants were catalogued. Our pipeline for variant calling reliably detected RGN-induced mutations at on-targets. When excluding these on-target modifications, variants were detected in edited lines at frequencies similar to those previously reported for spontaneous mutations. In further analyses, we did not find any evidence for an origin of these variants from RGN activity. Our data are thus consistent with high specificity of SpCas9. In contrast, we detected genomic reorganization events upon editing at two complex loci, RPP1 and RPP7, encompassing multiple homologous genes, and also identified an allele by WGS that had escaped detection by amplicon sequencing. We conclude that, while off-targets may efficiently be avoided by selection of specific gRNAs, on-target modifications may be more extensive than intended, especially at complex loci and/or during multiplexing.

plant biology↗

A cellulose synthase interactome uncovers BAG proteins as regulators of cellulose synthase homeostasis

Cellulose synthase complexes build the load-bearing cellulose microfibrils of plant cell walls, yet how the abundance of their catalytic CELLULOSE SYNTHASE A (CESA) subunits is maintained remains unclear. Here, we used multi-bait TurboID proximity labelling with ten cellulose-synthesis-associated baits and six subcellular controls to define a high-confidence cellulose synthase neighbourhood. Stringent spatial and recurrence-based filtering yielded a core network of 119 interactions among 44 proteins and identified three members of the conserved Bcl-2-associated athanogene (BAG) family as previously unrecognised regulators of cellulose synthase homeostasis. BAG1-3 associated with primary-wall CESAs in reciprocal proximity-labelling experiments. Arabidopsis bag mutants showed reduced cellulose accumulation, hypersensitivity to cellulose-synthesis inhibitors, and markedly decreased CESA protein abundance without corresponding changes in CESA transcript levels. Loss of BAG function also increased the accumulation of CESA6 in vacuolar compartments. These findings identify BAG proteins as previously unrecognised regulators of cellulose synthase homeostasis and link a conserved proteostasis-associated protein family to plant cell wall biosynthesis. More broadly, the study establishes multi-bait proximity labelling, combined with cell location-specific controls, as a strategy for resolving dynamic protein networks whose components traffic through multiple subcellular compartments.

plant biology↗