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bioRxiv · 10.1101/2025.02.08.637214

Igf2 regulates early postnatal DPP4+ preadipocyte pool expansion

Altun, I.·Walia, S.·Yan, X.·Singh, I.·Karlina, R.·Miok, V.·Kang, L.·Ho Diep Vo, K.·Lutter, D.·Perrochi, F.·Ussar, S.
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Abstract

Adipose tissue is rapidly expanding early in life. Identifying the queues facilitating this process will advance our understanding of metabolically healthy obesity. Single-cell RNA sequencing revealed compositional differences between pre-wean and adult murine subcutaneous adipose tissue. We identify a dipeptidyl peptidase-4 (Dpp4) positive precursor population in pre-wean mice residing at the reticular interstitium of subcutaneous adipose tissue expressing insulin growth factor 2 (Igf2). We show that IGF2 drives proliferation rather than differentiation in these cells. Moreover, loss of Igf2 in Dpp4+ progenitor cells promotes adipogenesis. We show that expression of Igf2 at a limited timeframe during development promotes preadipocyte expansion.

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Altun, I., Walia, S., Yan, X., Singh, I., Karlina, R., Miok, V., Kang, L., Ho Diep Vo, K., Lutter, D., Perrochi, F., Ussar, S.. 2025-02-08. Igf2 regulates early postnatal DPP4+ preadipocyte pool expansion. https://doi.org/10.1101/2025.02.08.637214

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