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bioRxiv · 10.1101/2025.02.06.636785

CD4+ Mucosal-associated Invariant T (MAIT) cells express highly diverse T cell receptors

Kaur, R.·Mehanna, N.·Pradhan, A.·Xie, D.·Li, K.·Aube, J.·Rosati, B.·Carlson, D.·Vorkas, C. K.
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Abstract

Mucosal-associated invariant T cells are highly conserved innate-like T cells in mammals recognized for their high baseline frequency in human blood and cytotoxic effector functions during infectious diseases, autoimmunity, and cancer. While the majority of these cells express a conserved CD8{beta}+ TRAV1-2 T cell receptor recognizing microbially-derived Vitamin B2 intermediates presented by the evolutionarily conserved major histocompatibility complex I-related molecule, MR1, there is an emerging appreciation for diverse subsets that may be selected for in humans with distinct functions, including subpopulations that co-express CD4. Prior work has not examined T cell receptor (TCR) heterogeneity in CD4+ MAIT cells, largely due to bias of identifying human MAIT cells as CD8+ TRAV1-2+ cells. In this study, we adopted an unbiased single-cell TCR-sequencing approach of total MR1-5-OP-RU-tetramer-reactive T cells and discovered that CD4+ MAIT cells express highly diverse TRAV1-2 negative TCRs. To specifically characterize this TCR repertoire, we analyzed VDJ sequences of single MR1-5-OP-RU tetramer+ MAIT cells across two datasets and identified distinct TCR usage among CD4+ MAIT cells including TRAV21, TRAV8 (TRAV8-1, TRAV8-2, TRAV8-3), and TRAV12 families (TRAV12-2, TRAV12-3), as well as more variable J chain and CDR3 sequences. Non-TRAV1-2 MAIT cell TCRs were also enriched after in vitro expansion, including with Mycobacterial tuberculosis. These results indicate that mature human CD4+ MAIT cells adopt distinct TCR usage from the canonical TRAV1-2+ CD8+ subset and suggest that alternative MR1 ligands in addition to riboflavin intermediates may select them.

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Kaur, R., Mehanna, N., Pradhan, A., Xie, D., Li, K., Aube, J., Rosati, B., Carlson, D., Vorkas, C. K.. 2025-02-08. CD4+ Mucosal-associated Invariant T (MAIT) cells express highly diverse T cell receptors. https://doi.org/10.1101/2025.02.06.636785

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