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bioRxiv · 10.1101/2025.02.04.636392

Effects of One-week Intake of Different Edible Oils on the Urinary Proteome of Rats

Su, Y.·Gao, Y.
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Abstract

ObjectiveTo explore the effects of different edible oils on the rat body by analyzing the changes in the urinary proteome and post-translational modifications of rats after one-week intake of olive oil, butter, lard, hydrogenated vegetable oil, and rapeseed oil. MethodsSix parallel experiments were set up using male Wistar rats. Group A served as the control group, while groups B - F were administered different edible oils. The daily intakes were calculated respectively according to the "2015 - 2020 Dietary Guidelines for Americans" and the "Dietary Guidelines for Chinese Residents". Urine samples were collected after one week and identified by label - free quantitative proteomics technology of high - performance liquid chromatography - tandem mass spectrometry (LC - MS/MS). Differentially expressed proteins and differential post - translational modifications in the urinary proteome were screened for functional analysis. ResultsAfter the intake of different types of edible oils, rats produced a large number of proteins and biological pathways related to metabolism, but there were few common differentially expressed proteins among different groups. In addition, the olive oil group and the butter group were enriched with many biological pathways related to the nervous system, and the rapeseed oil group produced more differentially expressed proteins and biological pathways related to immunity. ConclusionThe urinary proteome of rats showed significant changes after one-week intake of edible oils, and the effects of various edible oils on the rat urinary proteome were different from each other. This effect is comprehensive and multi-dimensional at the level of the rat body. The changes in post-translational modifications of the proteome were relatively small.

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Su, Y., Gao, Y.. 2025-02-05. Effects of One-week Intake of Different Edible Oils on the Urinary Proteome of Rats. https://doi.org/10.1101/2025.02.04.636392

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