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bioRxiv · 10.1101/2025.01.31.636009

Chromato-kinetic fingerprinting enables multiomic digital counting of single disease biomarker molecules

Banerjee, P.·Ray, S.·Dai, L.·Sandford, E.·Chatterjee, T.·Mandal, S.·Siddiqui, J.·Tewari, M.·Walter, N. G.
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Abstract

Early and personalized intervention in complex diseases requires robust molecular diagnostics, yet the simultaneous detection of diverse biomarkers--microRNAs (miRNAs), mutant DNAs, and proteins--remains challenging due to low abundance and preprocessing incompatibilities. We present Biomarker Single-molecule Chromato-kinetic multi-Omics Profiling and Enumeration (Bio-SCOPE), a next-generation, triple-modality, multiplexed detection platform that integrates both chromatic and kinetic fingerprinting for molecular profiling through digital encoding. Bio-SCOPE achieves femtomolar sensitivity, single-base mismatch specificity, and minimal matrix interference, enabling precise, parallel quantification of up to six biomarkers in a single sample with single-molecule resolution. We demonstrate its versatility in accurately detecting low-abundance miRNA signatures from human tissues, identifying upregulated miRNAs in the plasma of prostate cancer patients, and measuring elevated interleukin-6 (IL-6) and hsa-miR-21 levels in cytokine release syndrome patients. By seamlessly integrating multiomic biomarker panels on a unified, high-precision platform, Bio-SCOPE provides a transformative tool for molecular diagnostics and precision medicine.

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Banerjee, P., Ray, S., Dai, L., Sandford, E., Chatterjee, T., Mandal, S., Siddiqui, J., Tewari, M., Walter, N. G.. 2025-02-04. Chromato-kinetic fingerprinting enables multiomic digital counting of single disease biomarker molecules. https://doi.org/10.1101/2025.01.31.636009

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