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bioRxiv · 10.1101/2025.01.31.635967

Pupillometry Reveals Autonomic Adjustments During Diving Reflex in Face Immersion Apnea

Rizzuto, V.·Montanari, R.·Mesin, L.·Bortolozzo-Gleich, H.·Laurino, M.·Bonneh, Y.·Yellin, D.·Laganovska, G.·Vanags, J.·Gemignani, A.
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Abstract

The human diving reflex is an innate cardiorespiratory adjustment triggered during apnea that concurrently activates both sympathetic and parasympathetic branches of the autonomic nervous system to maintain physiological stability under stress. Although pupil dilation and constriction are antagonistically regulated by these branches, the effect of the diving reflex on pupil diameter oscillations, known as hippus, remains unexplored. Here, we compared hippus in healthy participants during breathing or apnea either with (Wet) or without (Dry) facial immersion in cold water. In both apneic conditions, hippus exhibited reduced power in the low-frequency band (< 0.25 Hz). Notably, during Wet apnea, we observed a reallocation of power towards higher frequencies (> 0.25 Hz) and increased entropy of fluctuations, indicating a shift in autonomic balance and greater signal complexity during the diving reflex. This pilot study highlights pupil dynamics as a sensitive and non-invasive probe of autonomic adjustments underlying the human diving reflex.

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Rizzuto, V., Montanari, R., Mesin, L., Bortolozzo-Gleich, H., Laurino, M., Bonneh, Y., Yellin, D., Laganovska, G., Vanags, J., Gemignani, A.. 2025-02-06. Pupillometry Reveals Autonomic Adjustments During Diving Reflex in Face Immersion Apnea. https://doi.org/10.1101/2025.01.31.635967

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