Reconstructing Sordida subcomplex (Hemiptera, Reduviidae, Triatominae) phylogeny across species distribution range

Burgueno-Rodriguez, G.; Nattero, J.; Rios, N.; Piccinali, R. V.; Carbajal-de-la-Fuente, A. L.; Panzera, F.; Sansoni-Ruidiaz, B. A.; Lopes, C. M.; Lobbia, P. A.; Rojas-de-Arias, A.; Cavallo, M. J.; Rodriguez, C. S.; Lorite, P.; Vega-Gomez, M. C.; Rolon, M.; Pita, S.

doi:10.1101/2025.01.30.635797

bioRxiv · 10.1101/2025.01.30.635797

Reconstructing Sordida subcomplex (Hemiptera, Reduviidae, Triatominae) phylogeny across species distribution range

Burgueno-Rodriguez, G.·Nattero, J.·Rios, N.·Piccinali, R. V.·Carbajal-de-la-Fuente, A. L.·Panzera, F.·Sansoni-Ruidiaz, B. A.·Lopes, C. M.·Lobbia, P. A.·Rojas-de-Arias, A.·Cavallo, M. J.·Rodriguez, C. S.·Lorite, P.·Vega-Gomez, M. C.·Rolon, M.·Pita, S.

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Abstract

The conformation of the Sordida subcomplex has been a topic of prolonged debate, with diverse methodological approaches employed to discern its constituent species. Up to now, Triatoma sordida, T. garciabesi and T. rosai comprise part of this subcomplex. Distinguishing and identifying these three species pose significant challenges due to their pronounced morphological similarity, overlapping distributions, and presence of natural hybrids. This study aims to uncover the genetic diversity and geographic spread of these three species by analyzing a mitochondrial cytochrome b gene fragment and complementing it with chromosomal studies across natural populations from an extensive geographical range, including Argentina, Bolivia, Brazil, and Paraguay. Phylogenetic analyses revealed genetic distances that suggest the presence of at least six putative species, rather than the three currently recognized. The present findings underscore the potency and significance of molecular analyses from natural populations for species identification and highlight the limitations of morphology in classifying Triatominae species.

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Burgueno-Rodriguez, G., Nattero, J., Rios, N., Piccinali, R. V., Carbajal-de-la-Fuente, A. L., Panzera, F., Sansoni-Ruidiaz, B. A., Lopes, C. M., Lobbia, P. A., Rojas-de-Arias, A., Cavallo, M. J., Rodriguez, C. S., Lorite, P., Vega-Gomez, M. C., Rolon, M., Pita, S.. 2025-02-05. Reconstructing Sordida subcomplex (Hemiptera, Reduviidae, Triatominae) phylogeny across species distribution range. https://doi.org/10.1101/2025.01.30.635797

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