Gut-Brain Hydraulics: Brain motion and CSF circulation is driven by mechanical coupling with the abdomen

Garborg, C. S.; Ghitti, B.; Zhang, Q.; Riccotta, J.; Frank, N.; Mueller, S.; Greenawalt, D.; Turner, K.; Kedarasetti, R.; Mostafa, M.; Lee, H.; Costanzo, F.; Drew, P. J.

doi:10.1101/2025.01.30.635779

bioRxiv · 10.1101/2025.01.30.635779

Gut-Brain Hydraulics: Brain motion and CSF circulation is driven by mechanical coupling with the abdomen

Garborg, C. S.·Ghitti, B.·Zhang, Q.·Riccotta, J.·Frank, N.·Mueller, S.·Greenawalt, D.·Turner, K.·Kedarasetti, R.·Mostafa, M.·Lee, H.·Costanzo, F.·Drew, P. J.

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Abstract

The brain moves within the skull, but the drivers and function of this motion are not understood. We visualized brain motion relative to the skull in awake head-fixed mice using high-speed, multi-plane two-photon microscopy. Brain motion was primarily rostrally and laterally directed, and was tightly correlated with locomotion, but not with respiration or the cardiac cycle. Electromyography recordings in abdominal muscles and microCT reconstructions of the trunk and spinal vasculature showed that brain motion was driven by abdominal muscle contractions that activate a hydraulic-like vascular connection between the nervous system and the abdominal cavity. Externally-applied abdominal pressure generated brain motion similar to those seen during abdominal muscle contractions. Simulations showed that brain motion drives substantial volumes of interstitial fluid through and out of the brain (at volumetric rates several times higher than production) into the subarachnoid space, in the opposite direction of fluid flow seen during sleep. The brain is hydraulically linked to the abdominal compartment, and fluid flow in the brain is coupled to body movements, providing a mechanism by which the mechanics of the viscera directly impact brain health.

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Garborg, C. S., Ghitti, B., Zhang, Q., Riccotta, J., Frank, N., Mueller, S., Greenawalt, D., Turner, K., Kedarasetti, R., Mostafa, M., Lee, H., Costanzo, F., Drew, P. J.. 2025-02-01. Gut-Brain Hydraulics: Brain motion and CSF circulation is driven by mechanical coupling with the abdomen. https://doi.org/10.1101/2025.01.30.635779

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