SPECTRE-Plex: an automated, fast, high-resolution-enabled approach for multiplexed cyclic imaging and tissue spatial analysis

Anderson, M. D.; Plone, A.; La, J.; Wong, M.; Raghunathan, K.; Silvester, J. A.; Thiagarajah, J. R.

doi:10.1101/2025.01.30.635753

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bioRxiv · 10.1101/2025.01.30.635753

SPECTRE-Plex: an automated, fast, high-resolution-enabled approach for multiplexed cyclic imaging and tissue spatial analysis

Anderson, M. D.·Plone, A.·La, J.·Wong, M.·Raghunathan, K.·Silvester, J. A.·Thiagarajah, J. R.

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Abstract

Mapping the spatial organization of tissues is critical to understanding organ biology in health and disease. Developments in multiplexed antibody-based, fluorescence labelling methods have provided unique insights into tissue microenvironments. However, many current methods have a variety of limitations that reduce their practical utilization. To address this, we developed SPECTRE-Plex; an end-to-end solution based on a series of methods that significantly improve speed, automation, and resolution of cyclic multiplex immunofluorescence imaging.

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Anderson, M. D., Plone, A., La, J., Wong, M., Raghunathan, K., Silvester, J. A., Thiagarajah, J. R.. 2025-02-05. SPECTRE-Plex: an automated, fast, high-resolution-enabled approach for multiplexed cyclic imaging and tissue spatial analysis. https://doi.org/10.1101/2025.01.30.635753

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