Sequential oxidation of L-lysine by a non-heme iron hydroxylase

Reynolds, E. S.; Smith, T. G.; Rama Damodaran, A.; Bhagi-Damodaran, A.

doi:10.1101/2025.01.27.635104

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bioRxiv · 10.1101/2025.01.27.635104

Sequential oxidation of L-lysine by a non-heme iron hydroxylase

Reynolds, E. S.·Smith, T. G.·Rama Damodaran, A.·Bhagi-Damodaran, A.

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Abstract

2-oxoglutarate-dependent non-heme iron hydroxylases offer a direct route to functionalizing C(sp3)-H bonds across a diverse range of substrates, making them prime candidates for chemoenzymatic synthetic strategies. We demonstrate the ability of a non-heme iron L-lysine dioxygenase to perform sequential oxidation and computationally explore structural elements that promote this reactivity.

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Reynolds, E. S., Smith, T. G., Rama Damodaran, A., Bhagi-Damodaran, A.. 2025-01-28. Sequential oxidation of L-lysine by a non-heme iron hydroxylase. https://doi.org/10.1101/2025.01.27.635104

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