Modification of Non-photochemical Quenching Pathways in the C4 Model Plant Setaria viridis Revealed Shared and Unique Photoprotection Mechanisms as Compared to C3 Plants

Milburn, G.; Morris, C. M.; Kosola, E.; Patel-Tupper, D.; Liu, J.; Pham, D. H.; Acosta-Gamboa, L.; Stone, W. D.; Pardi, S.; Hillman, K.; McHargue, W. E.; Becker, E.; Kang, X.; Sumner, J.; Bailey, C.; Thielen, P. M.; Jander, G.; Kane, C. N.; McAdam, S. A. M.; Lawton, T. J.; Nusinow, D. A.; Zhang, F.; Gore, M. A.; Cheng, J.; Niyogi, K. K.; Zhang, R.

doi:10.1101/2025.01.12.632622

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bioRxiv · 10.1101/2025.01.12.632622

Modification of Non-photochemical Quenching Pathways in the C4 Model Plant Setaria viridis Revealed Shared and Unique Photoprotection Mechanisms as Compared to C3 Plants

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Abstract

Light is essential for photosynthesis; however, excess light can increase the accumulation of photoinhibitory reactive oxygen species that reduce photosynthetic efficiency. Plants have evolved photoprotective non-photochemical quenching (NPQ) pathways to dissipate excess light energy. In tobacco and soybean (C3 plants), overexpression of three NPQ genes, violaxanthin de-epoxidase (VDE), Photosystem II Subunit S (PsbS), and zeaxanthin epoxidase (ZEP), hereafter VPZ, resulted in faster NPQ induction and relaxation kinetics, and increased crop yields in field conditions. NPQ is well-studied in C3 plants; however, NPQ and the translatability of the VPZ approach in C4 plants is poorly understood. The green foxtail Setaria viridis is an excellent model to study photosynthesis and photoprotection in C4 plants. To understand the regulation of NPQ and photosynthesis in C4 plants, we performed transient overexpression in Setaria protoplasts and generated (and employed) stable transgenic Setaria plants overexpressing one of the three Arabidopsis NPQ genes or all three NPQ genes (AtVPZ lines). Overexpressing (OE) AtVDE and AtZEP in Setaria produced similar results as in C3 plants, with increased or reduced zeaxanthin (thus NPQ), respectively. However, overexpressing AtPsbS appeared to be challenging in Setaria, with largely reduced NPQ in protoplasts and under-represented homozygous AtPsbS-OE lines, potentially due to competitive and tight heterodimerization of AtPsbS and SvPsbS proteins. Furthermore, Setaria AtVPZ lines had increased zeaxanthin, faster NPQ induction, higher NPQ level, but slower NPQ relaxation. Despite this, AtVPZ lines had improved growth as compared to wildtype under several conditions, especially high temperatures, which is not related to the faster relaxation of NPQ but may be attributable to increased zeaxanthin and NPQ in C4 plants. Our results identified shared and unique characteristics of the NPQ pathway in C4 model Setaria as compared to C3 plants and provide insights to improve C4 crop yields under fluctuating environmental conditions.

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Milburn, G., Morris, C. M., Kosola, E., Patel-Tupper, D., Liu, J., Pham, D. H., Acosta-Gamboa, L., Stone, W. D., Pardi, S., Hillman, K., McHargue, W. E., Becker, E., Kang, X., Sumner, J., Bailey, C., Thielen, P. M., Jander, G., Kane, C. N., McAdam, S. A. M., Lawton, T. J., Nusinow, D. A., Zhang, F., Gore, M. A., Cheng, J., Niyogi, K. K., Zhang, R.. 2025-01-15. Modification of Non-photochemical Quenching Pathways in the C4 Model Plant Setaria viridis Revealed Shared and Unique Photoprotection Mechanisms as Compared to C3 Plants. https://doi.org/10.1101/2025.01.12.632622

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