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bioRxiv · 10.1101/2025.01.12.632472

Catalytic degradation of circulating targets with FcRn-mediated cycling LYTACs

Abstract

Circulating proteins are common targets for the discovery of occupancy-based inhibitors including monoclonal antibodies. Effective inhibition of target pathogenicity with blocking approaches, however, is often challenged by target parameters that lead to insufficient occupancy and/or incomplete pharmacology limited by only single site binding. Extracellular targeted protein degradation approaches, such as lysosomal targeting chimeras (LYTACs), offer an opportunity to minimize these challenges by an event-driven mechanism that selectively, thoroughly and irreversibly eliminates drivers of disease. First generation LYTACs, designed to traffic to the lysosome, show limited durability since the therapeutic is degraded along with the target protein of interest. Here we describe cataLYTACs, which overcome this limitation by combining stabilized asialoglycoprotein (ASGPR) ligands, pH-sensitive target binding and recycling via the neonatal Fc receptor (FcRn). These cataLYTACs degraded superstoichiometric levels of a target protein, IgE, in vitro and demonstrated deep and sustained clearance of human IgE in mouse models. In non-human primates, cataLYTACs resulted in >98% clearance of circulating endogenous IgE for 2 weeks and outperformed the standard of care blocking antibody, omalizumab (Xolair(R)), in both free IgE elimination and duration of action. CataLYTACs represent a new therapeutic modality for a wide range of disease states driven by circulating factors, with the potential for superior efficacy and duration of action compared to traditional inhibitors.

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BibTeXRIS

Kougentakis, C., Shurtleff, M. J., Lieser, R. M., Chen, T., Collins, C. L., Johnson, I. E., Gu, S., Akama, K. T., Doran, K. H., Fung, C. K., Hildebrandt, D. H., Khaleghi, R., Li, D., Rank, S., Tran, T.-T., Yau, R., Holly, R. W., Langsdorf, C., Lindhout, D. A., McWhirter, S. M., Iwig, J. S., Lewis, J. G., Staben, S. T., Turtle, E. D., Lind, N. A.. 2025-01-13. Catalytic degradation of circulating targets with FcRn-mediated cycling LYTACs. https://doi.org/10.1101/2025.01.12.632472

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