Impact of Low-Dose Narrowband UV Radiation on Skin Tissue and Mast Cells in a Canine Model

Maeda, Y.; Kubota, S.; Kawarai, S.; Okuyama, H.; Kamimura, M.; Ono, M.; Nishida, M.; Moriya, S.; Onoda, Y.; Tsurumoto, T.; Nagata, K.; Ansai, S.-i.; Fujikawa, Y.; Tsukamoto, A.

doi:10.1101/2025.01.01.631029

bioRxiv · 10.1101/2025.01.01.631029

Impact of Low-Dose Narrowband UV Radiation on Skin Tissue and Mast Cells in a Canine Model

Maeda, Y.·Kubota, S.·Kawarai, S.·Okuyama, H.·Kamimura, M.·Ono, M.·Nishida, M.·Moriya, S.·Onoda, Y.·Tsurumoto, T.·Nagata, K.·Ansai, S.-i.·Fujikawa, Y.·Tsukamoto, A.

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Abstract

Narrowband ultraviolet radiation (NB-UVR) therapy is widely utilized in dermatology worldwide. While the immunosuppressive effects of ultraviolet radiation are well-documented, its non-inflammatory effects remain poorly understood. This study evaluated the effects of sub-minimal erythema dose (MED) narrowband ultraviolet radiation using a canine model. The effects of narrowband-ultraviolet B (310 nm) and A2 wavelengths (320 and 330 nm) were compared. Initially, we demonstrated the therapeutic potential of NB-UVR in dogs with spontaneous atopic dermatitis (n = 6, p < 0.05). Following 300 mJ/cm{superscript 2} sub-MED ultraviolet radiation exposure, intradermal skin testing, skin mast cell counts, and RNA sequencing analysis were conducted. Additionally, cell viability (WST assay) and apoptosis (DAPI staining) were assessed in vitro using a canine mast cell line. Ultraviolet radiation exposure significantly reduced intradermal skin test scores across all wavelengths and decreased skin mast cell counts at 310 nm (n = 3, p < 0.05). In vitro, cell viability was significantly reduced at 310 and 320 nm (p < 0.05), and 310 nm irradiation specifically increased apoptotic cell numbers (p < 0.05). RNA sequencing identified 71 differentially expressed genes at 320 nm, with enrichment analysis highlighting muscle cell components and functions, potentially associated with arrector pili muscle activity. These findings suggest that 310 nm irradiation induces apoptosis, while 320 nm elicits non-inflammatory modulatory effects, highlighting distinct wavelength-specific therapeutic mechanisms of narrowband ultraviolet radiation.

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Maeda, Y., Kubota, S., Kawarai, S., Okuyama, H., Kamimura, M., Ono, M., Nishida, M., Moriya, S., Onoda, Y., Tsurumoto, T., Nagata, K., Ansai, S.-i., Fujikawa, Y., Tsukamoto, A.. 2025-01-02. Impact of Low-Dose Narrowband UV Radiation on Skin Tissue and Mast Cells in a Canine Model. https://doi.org/10.1101/2025.01.01.631029

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