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bioRxiv · 10.1101/2024.12.30.630730

Layilin Regulates Treg Motility and Suppressive Capacity in Skin

Gouirand, V.·Clancy, S.·Macon, C.·Valle, J.·Pauli, M.·Truong, H.-A.·Cohen, J.·Kinet, M.·Lowe, M. M.·Lord, S. J.·Skruber, K.·Harris, H.·Kim, E.·Neuhaus, I.·Reif, K.·Zarrin, A. A.·Mullins, R. D.·Rosenblum, M.
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Abstract

Regulatory T cells (Tregs) are essential for maintaining immune tolerance in both lymphoid and non-lymphoid tissues. We discovered that layilin, a C-type lectin receptor, is predominantly expressed on Tregs in skin. Layilin was highly expressed on a subset of clonally expanded effector Tregs in both healthy and psoriatic skin. Layilin expressing Tregs exhibited a transcriptional profile indicative of enhanced adhesion. Deletion of layilin in Tregs in vivo resulted in significantly attenuated skin inflammation. Mechanistically, layilin enhanced Treg adhesion via modulation of LFA-1, resulting in distinct cytoskeletal alterations consistent with enhanced focal adhesion and lamellipodia formation. Taken together, we define layilin as a critical regulator of Treg suppressive capacity through modulating motility and adhesion in a non-lymphoid tissue.

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Gouirand, V., Clancy, S., Macon, C., Valle, J., Pauli, M., Truong, H.-A., Cohen, J., Kinet, M., Lowe, M. M., Lord, S. J., Skruber, K., Harris, H., Kim, E., Neuhaus, I., Reif, K., Zarrin, A. A., Mullins, R. D., Rosenblum, M.. 2024-12-30. Layilin Regulates Treg Motility and Suppressive Capacity in Skin. https://doi.org/10.1101/2024.12.30.630730

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