Barcoding-inferred biodiversity of shallow-water Indo-Pacific demosponges

Erpenbeck, D.; Galitz, A.; Berumen, M. L.; Büttner, G.; Debitus, C.; Dirnberger, M.; Ekins, M.; Hall, K.; Namuth, L.; Petek, S.; Rahnamae, N.; Reddy, M. M.; Rettenberger, D.; Ries, S. R.; Schätzle, S.; Schönberg, C. H.; Setiawan, E.; van der Sprong, J.; Thomas, O. P.; Tirumalasetty, V.; de Voogd, N. J.; Voigt, O.; Hooper, J. N.; Wörheide, G.

doi:10.1101/2024.12.29.630654

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bioRxiv · 10.1101/2024.12.29.630654

Barcoding-inferred biodiversity of shallow-water Indo-Pacific demosponges

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Abstract

AimThe Indo-Pacific is the worlds largest marine biogeographic region. It is characterised by different degrees of connectivity among its subregions, and harbours the majority of demosponge species currently known to science. Comparisons between several regional sponge faunas have been undertaken in the past, mostly based on identifying the sponge species morphologically. The Sponge Barcoding Project, in tandem with other regional DNA taxonomy campaigns, provides one of the largest DNA-based taxonomic data collections from sponges of the Indo-Pacific. Here, we utilise the sponge barcoding data in the largest molecular biodiversity study of sponges to date, which reveals patterns of shallow-water demosponge faunal connectivity, endemism, and distribution in the Indo-Pacific with a level of resolution unavailable in prior morphology-based studies. LocationDemosponge specimens in this study cover 13 marine provinces of the Indo-Pacific, from the Red Sea to South East Polynesia. MethodsWe classified demosponge barcodes using the ribosomal subunit (28S rDNA) of 1,910 sponge samples into molecular operational taxonomic units (MOTUs). MOTU composition of the 13 marine provinces was compared based on Jaccard and Sorenson dissimilarities, and other biodiversity indices. ResultsOur data corroborated high levels of endemism among demosponges. Faunal overlaps were revealed between the Red Sea and the Gulf, which displayed relatively small connectivity with other marine provinces of the Western Indian Ocean. In the Western Indian Ocean, we observed a strong faunistic boundary to the Central Indo-Pacific. The Polynesian sponge faunas were comparatively isolated marine provinces of the Central Indo-Pacific. Main conclusionsOur data corroborate case studies on sponges that generally reject the presence of cosmopolitan or otherwise widespread sponge species, instead revealing high levels of regional endemism. This is consistent with similar observations and hypotheses in other marine invertebrates. Connectivity among Indo-Pacific marine provinces differs for demosponges in many aspects from that of other marine taxa, such as corals and fishes, probably due to their shorter pelagic larval phase.

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Erpenbeck, D., Galitz, A., Berumen, M. L., Büttner, G., Debitus, C., Dirnberger, M., Ekins, M., Hall, K., Namuth, L., Petek, S., Rahnamae, N., Reddy, M. M., Rettenberger, D., Ries, S. R., Schätzle, S., Schönberg, C. H., Setiawan, E., van der Sprong, J., Thomas, O. P., Tirumalasetty, V., de Voogd, N. J., Voigt, O., Hooper, J. N., Wörheide, G.. 2024-12-29. Barcoding-inferred biodiversity of shallow-water Indo-Pacific demosponges. https://doi.org/10.1101/2024.12.29.630654

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