Generalists in a Specialist World: Vibrio-Phages with Broad Host Range

Bernard, C.; Labreuche, Y.; Diarra, C.; Daszkowski, P.; Cahier, K.; Goudenege, D.; Lamarche, M.; Whitfield, G. B.; Lang, M.; Valencia, J.; Groseille, J.; Piel, D.; Brun, Y. V.; Rocha, E.; Le Roux, F.

doi:10.1101/2024.12.24.630238

bioRxiv · 10.1101/2024.12.24.630238

Generalists in a Specialist World: Vibrio-Phages with Broad Host Range

Bernard, C.·Labreuche, Y.·Diarra, C.·Daszkowski, P.·Cahier, K.·Goudenege, D.·Lamarche, M.·Whitfield, G. B.·Lang, M.·Valencia, J.·Groseille, J.·Piel, D.·Brun, Y. V.·Rocha, E.·Le Roux, F.

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Abstract

The host range of a bacteriophage--the diversity of hosts it can infect--is central to understanding phage ecology and applications. While most well-characterized phages have narrow host ranges, broad-host-range phages represent an intriguing component of marine ecosystems. The genetic and evolutionary mechanisms driving their generalism remain poorly understood. In this study, we analyzed Schizotequatroviruses and their Vibrio crassostreae hosts, collected from an oyster farm. Schizotequatroviruses exhibit broad host ranges, large genomes (~252 kbp) encoding 26 tRNAs, and conserved genomic organization interspersed with recombination hotspots. These recombination events, particularly in regions encoding receptor-binding proteins and antidefense systems, highlight their adaptability to host resistance. Notably, some lineages demonstrated receptor-switching between OmpK and LamB, showcasing their evolutionary flexibility. Despite their broad host range, Schizotequatroviruses were rare in the environment. Their scarcity could not be attributed to burst size, which was comparable to other phages in vitro, but may result from ecological constraints or fitness trade-offs, such as their preference for targeting generalist vibrios in seawater rather than the patho-phylotypes selected in oyster farms. Our findings clarify the genetic and ecological trade-offs shaping Schizotequatrovirus generalism and provide a foundation for future phage applications in aquaculture and beyond.

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Bernard, C., Labreuche, Y., Diarra, C., Daszkowski, P., Cahier, K., Goudenege, D., Lamarche, M., Whitfield, G. B., Lang, M., Valencia, J., Groseille, J., Piel, D., Brun, Y. V., Rocha, E., Le Roux, F.. 2024-12-24. Generalists in a Specialist World: Vibrio-Phages with Broad Host Range. https://doi.org/10.1101/2024.12.24.630238

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