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bioRxiv · 10.1101/2024.12.20.629258

The yeast CST and Polα/primase complexes act in concert to ensure proper telomere maintenance and protection

Calugaru, K.·Yu, E. Y.·Gonzalez-Rodriguez, N.·Coloma, J.·Lue, N.
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Abstract

Pol/primase, the polymerase that initiates DNA synthesis at replication origins, also completes the task of genome duplication by synthesizing the telomere C-strand under the control of the CST complex. Using cryo-EM structures of the human CST-Pol/primase-DNA complex as guides in conjunction with AlphaFold modeling, we identified structural elements in yeast CST and Pol/primase that promote complex formation. Mutating these structures in Candida glabrata Stn1, Ten1, Pri1 and Pri2 abrogated the stimulatory activity of CST on Pol/primase in vitro, supporting the functional relevance of the physical contacts in cryo-EM structures as well as the conservation of mechanisms between yeast and humans. Introducing these mutations into C. glabrata yielded two distinct groups of mutants. One group exhibited progressive, telomerase-dependent telomere elongation without evidence of DNA damage. The other manifested slow growth, telomere length heterogeneity, ssDNA accumulation and elevated C-circles, which are indicative of telomere deprotection. These telomere deprotection phenotypes are altered or suppressed by mutations in multiple DDR and DNA repair factors. We conclude that in yeast, the telomerase inhibition and telomere protection function previously ascribed to the CST complex are mediated jointly by both CST and Pol/primase, highlighting the critical importance of a replicative DNA polymerase in telomere regulation.

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Calugaru, K., Yu, E. Y., Gonzalez-Rodriguez, N., Coloma, J., Lue, N.. 2024-12-20. The yeast CST and Polα/primase complexes act in concert to ensure proper telomere maintenance and protection. https://doi.org/10.1101/2024.12.20.629258

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