A FRET-Based FLIM Method to Probe Membrane-Induced Alpha-Synuclein Aggregation in Neurons

Ivey, P. M. E.; Salem, A.; Min, S.; Qi, W.; Guzman Sosa, M.; Kinzer-Ursem, T. L.; Rochet, J.-C.; Webb, K. J.

doi:10.1101/2024.12.19.629536

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bioRxiv · 10.1101/2024.12.19.629536

A FRET-Based FLIM Method to Probe Membrane-Induced Alpha-Synuclein Aggregation in Neurons

Ivey, P. M. E.·Salem, A.·Min, S.·Qi, W.·Guzman Sosa, M.·Kinzer-Ursem, T. L.·Rochet, J.-C.·Webb, K. J.

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Abstract

1Parkinsons disease (PD) involves the aggregation of the protein alpha-synuclein, a process promoted by interactions with intracellular membranes. To study this phenomenon in neurons for the first time, we developed a fluorescence lifetime imaging (FLIM) method using Forster resonance energy transfer and self-quenching reporters, analyzed with a custom-built FLIM microscope. This method offers insights into aggregate formation in PD and can be broadly applied to probe protein-membrane interactions in neurons.

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Ivey, P. M. E., Salem, A., Min, S., Qi, W., Guzman Sosa, M., Kinzer-Ursem, T. L., Rochet, J.-C., Webb, K. J.. 2024-12-20. A FRET-Based FLIM Method to Probe Membrane-Induced Alpha-Synuclein Aggregation in Neurons. https://doi.org/10.1101/2024.12.19.629536

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