Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures

Blaszczyk, G. J.; Piscopo, V. E. C.; Goldsmith, T. M.; Chapleau, A.; Sirois, J.; Bernard, G.; Antel, J.; Durcan, T. M.

doi:10.1101/2024.12.18.629204

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bioRxiv · 10.1101/2024.12.18.629204

Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures

Blaszczyk, G. J.·Piscopo, V. E. C.·Goldsmith, T. M.·Chapleau, A.·Sirois, J.·Bernard, G.·Antel, J.·Durcan, T. M.

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Abstract

Cellular heterogeneity is a common issue in differentiation protocols of oligodendrocytes (OLs) from human induced pluripotent stem cells. Our previous work described a novel method to generate OLs and highlighted the presence of glial progenitors. Here, we unravel the glial heterogeneity and characterize the response of isolated subpopulations to differentiation. This study provides a novel tool for studying the dynamics of glial development in vitro and on a transcriptomic level.

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Blaszczyk, G. J., Piscopo, V. E. C., Goldsmith, T. M., Chapleau, A., Sirois, J., Bernard, G., Antel, J., Durcan, T. M.. 2024-12-20. Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures. https://doi.org/10.1101/2024.12.18.629204

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