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bioRxiv · 10.1101/2024.12.18.629076

An updated proteomic analysis of Drosophila hemolymph after bacterial infection

Rommelaere, S.·Schupfer, F.·Armand, F.·Hamelin, R.·Lemaitre, B.
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Abstract

Using an in-depth Mass Spectrometry based proteomics approach, we provide a comprehensivecharacterization of the hemolymphatic proteome of adult flies upon bacterial infection. We detected and quantified changes in abundance of several known immune regulators and effectors, including multiple antimicrobial peptides, peptidoglycan-binding proteins and serine proteases. Comparison to previously published transcriptomic analyses reveals a partial overlap with our dataset, indicating that many proteins released into the hemolymph upon infection may not be regulated at the transcript level. Among them, we identify a set of muscle-derived proteins released into the hemolymph upon infection. Finally, our analysis reveals that infection induces major changes in the abundance of proteins associated with mitochondrial respiration. This study uncovers a large number of previously undescribed proteins potentially involved in the immune response.

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Rommelaere, S., Schupfer, F., Armand, F., Hamelin, R., Lemaitre, B.. 2024-12-20. An updated proteomic analysis of Drosophila hemolymph after bacterial infection. https://doi.org/10.1101/2024.12.18.629076

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