DynaMAP: Dynamic Microbiome Abundance Profiling through high density optical mapping

Abakumov, S.; Ruppeka-Rupeika, E.; Chen, X.; Engelbrecht, M.; Mestdagh, I.; D'Huys, T.; Mlodzinska, A.; Leen, V.; Szymanek, A.; Badola, A.; Lukes, T.; Demuyser, T.; Hofkens, J.; Dedecker, P.; Bouwens, A.

doi:10.1101/2024.12.05.626436

bioRxiv · 10.1101/2024.12.05.626436

DynaMAP: Dynamic Microbiome Abundance Profiling through high density optical mapping

Abakumov, S.·Ruppeka-Rupeika, E.·Chen, X.·Engelbrecht, M.·Mestdagh, I.·D'Huys, T.·Mlodzinska, A.·Leen, V.·Szymanek, A.·Badola, A.·Lukes, T.·Demuyser, T.·Hofkens, J.·Dedecker, P.·Bouwens, A.

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Abstract

Microbial abundance profiling is rapidly becoming an essential method in biomedical research, though it is often costly and time intensive. We present DynaMAP, a rapid approach for microbiome profiling that uses single-molecule imaging to develop an optical map of metagenomic DNA. DynaMAP achieves strain-level taxonomic profiling without requiring base-by-base sequencing and with a one-day turnaround time. In doing so, it delivers microbiome profiling that is comparable to shotgun sequencing but operates more efficiently, strongly expanding the possibility for microbiome analysis.

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Abakumov, S., Ruppeka-Rupeika, E., Chen, X., Engelbrecht, M., Mestdagh, I., D'Huys, T., Mlodzinska, A., Leen, V., Szymanek, A., Badola, A., Lukes, T., Demuyser, T., Hofkens, J., Dedecker, P., Bouwens, A.. 2024-12-05. DynaMAP: Dynamic Microbiome Abundance Profiling through high density optical mapping. https://doi.org/10.1101/2024.12.05.626436

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