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bioRxiv · 10.1101/2024.12.02.626147

Retrotransposon LINE1 trans-regulatory differentiation process maintain embryonic stem cell identity

Ren, L.·Xu, W.·Teng, Y.·Hou, J.·Zheng, C.·Cai, J.
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Abstract

Long Interspersed Nuclear Elements-1s (LINE1s) are one of the >17% most abundant Retrotransposons in mammalian genomes. LINE1 RNA is high expression during early embryonic development, meanwhile is under tight epigenetic control. Some studies have confirmed LINE1 play a critical role in early embryo development and ESC identity. Previous studies focused on the role of LINE1 in cis-regulation, however, the function of L1 RNA in tans-regulation remains largely unknown. In this study, we performed a high-throughput proximity MARGI (pxMARGI) experiment found transposon LINE1 RNA trans-regulated prefer to young LINE1 subfamily, and in hybrid and non-hybrid two different manner repress ESC to dual progress of 2C-like cells and differentiation. In differentiation process, LINE1 RNA as a scaffold recruit polycomb core subunits combine three core pluripotent factors maintain ESC identity. In 2C process, LINE1 RNA in a sequence-specific manner recruit Kap1 to old L1 subfamily, and recruit ELL3 to RE 5 UTR maintain ESC self-renewal. Our data point to LINE1 transcriptions more detail trans-regulation mechanism to orchestrate developmental progression for the self-renewal of embryonic stem cells (ESCs). LINE1 RNA may be potential biomarker for noninvasive embryo selection.

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Ren, L., Xu, W., Teng, Y., Hou, J., Zheng, C., Cai, J.. 2024-12-04. Retrotransposon LINE1 trans-regulatory differentiation process maintain embryonic stem cell identity. https://doi.org/10.1101/2024.12.02.626147

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