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bioRxiv · 10.1101/2024.11.25.625123

Structure and mechanism of the RalGAP tumor suppressor complex

Rasche, R.·Klink, B. U.·Apken, L. H.·Michalke, E.·Chen, M.·Oeckinghaus, A.·Gatsogiannis, C.·Kuemmel, D.
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Abstract

The RalGAP (GTPase activating protein) complexes are negative regulators of the Ral GTPases and thus crucial components that counteract (oncogenic) Ras signaling. However, no structural information on the architecture of this tumor suppressor complex is available hampering a mechanistic understanding of its functionality. Here, we present a cryo-EM structure of RalGAP that reveals an extended 58 nm tetrameric architecture comprising two heterodimers of the RalGAP and RalGAP{beta} subunits. We show that the catalytic domain of RalGAP requires stabilization by a unique domain of RalGAP{beta}, providing the molecular basis for why RalGAP complexes are obligatory heterodimers. Formation of RalGAP tetramers is not required for activity in vitro, but essential for function of the complex in vivo. Structural analysis of RalGAP subunit variants reported in cancer patients suggests effects on complex formation and thus functional relevance in tumor development, emphasizing the significance of the obtained structural information for medical research.

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Rasche, R., Klink, B. U., Apken, L. H., Michalke, E., Chen, M., Oeckinghaus, A., Gatsogiannis, C., Kuemmel, D.. 2024-11-25. Structure and mechanism of the RalGAP tumor suppressor complex. https://doi.org/10.1101/2024.11.25.625123

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