Leg immobilization and subsequent recovery resistance training affect skeletal muscle angiogenesis related markers in young healthy adults regardless of prior resistance training experience

McIntosh, M. C.; Michel, J. M.; Godwin, J. S.; Plotkin, D. L.; Anglin, D. A.; Mattingly, M. L.; Agyin-Birikorang, A.; Kontos, N. J.; Baweja, H. S.; Stock, M. S.; Mobley, C. B.; Roberts, M. D.

doi:10.1101/2024.11.24.625075

bioRxiv · 10.1101/2024.11.24.625075

Leg immobilization and subsequent recovery resistance training affect skeletal muscle angiogenesis related markers in young healthy adults regardless of prior resistance training experience

McIntosh, M. C.·Michel, J. M.·Godwin, J. S.·Plotkin, D. L.·Anglin, D. A.·Mattingly, M. L.·Agyin-Birikorang, A.·Kontos, N. J.·Baweja, H. S.·Stock, M. S.·Mobley, C. B.·Roberts, M. D.

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Abstract

We recently reported that resistance trained (T, n=10) and untrained (UT, n=11) young adults experience vastus lateralis (VL) muscle atrophy following two weeks of disuse, and 8 weeks of recovery resistance training (RT) promotes VL hypertrophy in both participant cohorts. However, angiogenesis targets and muscle capillary number were not examined and currently no human studies that have sought to determine if disuse followed by recovery RT affects these outcomes. Thus, we examined whether disuse and/or recovery RT affected these outcomes. All participants underwent two weeks of left leg immobilization using locking leg braces and crutches followed by eight weeks (3d/week) of knee extensor focused progressive RT. VL biopsies were obtained at baseline (PRE), immediately after disuse (MID), and after RT (POST). Western blotting was used to assay angiogenesis markers and immunohistochemistry was performed in 16/21 participants to determine type I and II muscle fiber capillary number. Significant main effects of time (p<0.05) were observed for protein levels of VEGF (MID 0.100). Although disuse and recovery RT affect skeletal muscle angiogenesis-related protein targets, prior training history does not differentially affect these outcomes. NEW AND NOTEWORTHYThis is the first study to examine how limb immobilization and recovery resistance training affect molecular outcomes related to angiogenesis in younger adults with or without a prior training history. Regardless of resistance training history, the molecular responses are largely similar between participant cohorts and is suggestive of a reduced (pre-mid) and increased (mid-post) angiogenic response, with disuse and subsequent recovery resistance training.

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McIntosh, M. C., Michel, J. M., Godwin, J. S., Plotkin, D. L., Anglin, D. A., Mattingly, M. L., Agyin-Birikorang, A., Kontos, N. J., Baweja, H. S., Stock, M. S., Mobley, C. B., Roberts, M. D.. 2024-11-26. Leg immobilization and subsequent recovery resistance training affect skeletal muscle angiogenesis related markers in young healthy adults regardless of prior resistance training experience. https://doi.org/10.1101/2024.11.24.625075

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