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bioRxiv · 10.1101/2024.11.15.623645

Anti-HIV-1 B cell antigen receptor signaling and structure

Abstract

The B cell antigen receptor (BCR) complex, comprised of antigen recognition and signaling components, functions in initiating B cell activation. While structural studies have described BCR domain organization, gaps remain in our understanding of its antigen binding domain (Fab, fragment antigen-binding) disposition, and how antigen binding is sensed to initiate signaling. Here, we report antigen affinity and signaling of the immunoglobulin (Ig) class IgM and IgG BCRs and define conformational states of full-length BCRs of two human broadly neutralizing antibodies, the glycan-specific, heavy chain domain-swapped, I-shaped 2G12, and a canonical Y-shaped antibody, CH31, that recognizes the CD4-binding site on the HIV-1 Envelope protein (Env). The BCRs adopted the shapes (I or Y) of their respective soluble antibodies, and both Ig class of BCRs of the same specificity bound Env trimers with similar affinities. We observed antigen-valenc y dependent differential signaling by the 2G12 IgM and IgG BCRs with trimeric Envs. Cryo-electron microscopy of the 2G12 IgG and CH31 IgM BCRs revealed varied Fab orientations. Structural comparisons revealed hinge points and regions of flexibility in the BCRs suggesting a highly dynamic structure of the BCR complex. Taken together, our results provide an integrated understanding of BCR structure, conformation, antigen recognition and signaling, and provide the basis for understanding antigen induced BCR signal transmission. One Sentence SummaryCryo-electron microscopy structures of full-length B cell antigen receptor complex provide a novel dynamic BCR model and a basis for understanding antigen binding induced signal transmission.

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BibTeXRIS

Thakur, B., Alam, J., Cronin, K., Patel, P., Anasti, K., Pai Kane, A., Hossain, A., Do, H. N., Mansouri, K., Spence, T. N., Edwards, R. J., Janowska, K., Lella, M., Cook, A., Saunders, K. O., Gnanakaran, S., Haynes, B. F., Acharya, P., Alam, S. M.. 2024-11-15. Anti-HIV-1 B cell antigen receptor signaling and structure. https://doi.org/10.1101/2024.11.15.623645

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