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bioRxiv · 10.1101/2024.11.13.619586

A Novel C. elegans Model for Tau spreading Reveals Genes Critical for Endolysosomal Integrity and Seeded Tau Aggregation

Abstract

The spreading of Tau pathology is closely associated with the progression of neurodegeneration and cognitive decline in Alzheimers disease and other tauopathies. A key event in this process is the rupture of endolysosomal vesicles following the intercellular transfer of Tau aggregates, releasing the transferred Tau species into the cytosol where they can promote the aggregation of endogenous Tau. However, understanding of the cellular pathways involved in this process remains limited. In this study, we investigated cellular pathways that prevent endolysosomal vesicle rupture. We established a new C. elegans model of Tau spreading by introducing an mCherry-labelled, disease-associated aggregation-prone fragment of human Tau (F3{Delta}K281::mCh) into the six touch receptor neurons. F3{Delta}K281::mCh transgenic animals exhibited significant neurotoxicity and mechanosensory deficits due to the accumulation of this Tau fragment. In addition, its intercellular transmission compromised the endolysosomal system in receiving hypodermal cells. Using this model, we conducted an unbiased genome-wide RNAi screen and identified 59 genes critical for maintaining endolysosomal integrity. GO-term analysis revealed an enrichment of genes related to the ESCRT complex, the ubiquitin-proteasome system, mRNA splicing, and fatty acid metabolism. Silencing of selected conserved genes exacerbated seeded Tau aggregation in a human induced pluripotent stem cell (hiPSC)-derived cortical neuron model and triggered endolysosomal rupture in HEK293T cells, confirming the crucial role of endolysosomal damage in seeded Tau aggregation. Overall, this study discovered novel cellular pathways that safeguard endolysosomal integrity. These findings may guide the development of therapeutics that improve endolysosomal integrity to halt the progression of Tau pathology.

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BibTeXRIS

Sandhof, C. A., Martin, N., Tittelmeier, J., Schlueter, A., Pezzali, M., Schoendorf, D. C., Lange, T., Reinhardt, P., Ried, J. S., Liang, S., Uzunoglu, G., Gasparini, L., Jahn, T., Ehrnhoefer, D. E., Nussbaum-Krammer, C.. 2024-11-15. A Novel C. elegans Model for Tau spreading Reveals Genes Critical for Endolysosomal Integrity and Seeded Tau Aggregation. https://doi.org/10.1101/2024.11.13.619586

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