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bioRxiv · 10.1101/2024.11.10.622884

Ligand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry

Conoan Nieves, N. E.·Widom, J. R.
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Abstract

The Class II NAD+ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with {beta}-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg2+.

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Conoan Nieves, N. E., Widom, J. R.. 2024-11-11. Ligand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry. https://doi.org/10.1101/2024.11.10.622884

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