Olfactory bulb tracks breathing rhythms and place in freely behaving mice

Abstract

Vertebrates sniff to control the odor samples that enter their nose. These samples can not only help identify odorous objects, but also locations and events. However, there is no receptor for place or time. Therefore, to take full advantage of olfactory information, an animals brain must contextualize odor-driven activity with information about when, where, and how they sniffed. To better understand contextual information in the olfactory system, we captured the breathing and movements of mice while recording from their olfactory bulb. In stimulus- and task-free experiments, mice structure their breathing into persistent rhythmic states which are synchronous with statelike structure in ongoing neuronal population activity. These population states reflect a strong dependence of individual neuron activity on variation in sniff frequency, which we display using "sniff fields" and quantify using generalized linear models. In addition, many olfactory bulb neurons have "place fields" that display significant dependence of firing on allocentric location, which were comparable with hippocampal neurons recorded under the same conditions. At the population level, a mouses location can be decoded from olfactory bulb with similar accuracy to hippocampus. Olfactory bulb place sensitivity cannot be explained by breathing rhythms or scent marks. Taken together, we show that the mouse olfactory bulb tracks breathing rhythms and self-location, which may help unite internal models of self and environment with olfactory information as soon as that information enters the brain.