bioRxiv Science⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2024.11.06.622231

Lack of functional polyester-biodegrading potential in marine versus terrestrial environments evidenced by an innovative airbrushing technique

Contreras-Moll, A.·Obrador-Viel, T.·Molina, R. D. I.·Aguilo-Ferretjans, M. d. M.·Nogales, B.·Bosch, R.·Christie-Oleza, J.
View original source ↗Export metadata ↓

Abstract

Biodegradable plastics, primarily aliphatic polyesters, degrade to varying extents in different environments. However, the absence of easily implementable techniques for screening microbial biodegradation potential --coupled with the limitations of non-functional omics analyses-- has restricted comparative studies across diverse polymer types and ecosystems. In this study, we optimized a novel airbrushing method that facilitates functional analyses by simplifying the preparation of polyester-coated plates for biodegradation screening. By repurposing an airbrush kit, polyester microparticles (MPs) could be evenly sprayed onto solid media, enabling rapid detection of extracellular depolymerizing activity via clearing zone halos. This technique was effective in screening both isolated microbial cultures and natural environmental samples, demonstrating its versatility. The method was successfully applied across multiple environments, ranking the biodegradability of six polyesters, from most to least biodegradable: polycaprolactone (PCL), poly[(R)-3-hydroxybutyrate] (PHB), poly(butylene succinate) (PBS), poly(ethylene succinate) (PES), poly(lactic acid) (PLA), and poly(butylene adipate-co-terephthalate) (PBAT). Most notably, it revealed a consistent 1,000-fold higher biodegradation potential in terrestrial compared to marine environments. This approach offers a valuable tool for isolating novel polyester-degrading microbes with significant biotechnological potential, paving the way for improved plastic waste management solutions. SYNOPSISScreening polyester-degrading microbes has traditionally been challenging due to the difficulty of incorporating polyesters into solid media. This study introduces an innovative airbrush spraying method that simplifies the preparation of polyester MPs on solid media plates, making the screening process significantly more efficient. Using this method, we demonstrated the differential biodegradability of aliphatic polyesters across environments. The results revealed a stark contrast in biodegrading potential, with terrestrial ecosystems exhibiting substantially higher activity compared to marine environments. Abstract graphic O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=79 SRC="FIGDIR/small/622231v1_ufig1.gif" ALT="Figure 1"> View larger version (15K): org.highwire.dtl.DTLVardef@176883eorg.highwire.dtl.DTLVardef@eab2aforg.highwire.dtl.DTLVardef@792bbdorg.highwire.dtl.DTLVardef@126ab7d_HPS_FORMAT_FIGEXP M_FIG C_FIG

Explore related subjects

microbiologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Contreras-Moll, A., Obrador-Viel, T., Molina, R. D. I., Aguilo-Ferretjans, M. d. M., Nogales, B., Bosch, R., Christie-Oleza, J.. 2024-11-06. Lack of functional polyester-biodegrading potential in marine versus terrestrial environments evidenced by an innovative airbrushing technique. https://doi.org/10.1101/2024.11.06.622231

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

A population-scale landscape of the subgingival microbiome reveals divergent routes to periodontal dysbiosis

Periodontitis is an archetypical mucosal inflammatory disease in which microbiome dysbiosis at the tooth-epithelial interface interacts with host genetic and behavioral risk factors to drive immune-mediated tissue destruction. Although subgingival microbiome compositional shifts are thought to parallel disease severity, microbiome variation at the population-level and its relationship to periodontal clinical phenotypes and disease-modifying factors remain poorly defined. Here, we use unsupervised manifold learning to map the compositional landscape of the subgingival microbiome in 1,355 adults spanning periodontal health to severe periodontitis. We identified eight latent microbiome states organized along a branching continuum from eubiosis to dysbiosis. An intermediate microbial configuration marked ecological destabilization and bifurcation into two distinct periodontitis-associated dysbiotic trajectories, distinguished by links to gingival inflammation and smoking. Although the microbiome trajectories broadly tracked periodontal destruction, a minority of individuals showed discordant microbiome-clinical phenotypes, with some individuals with periodontitis retaining otherwise eubiotic microbiomes enriched for low-abundance pathobionts, while some cases of health or mild disease had highly dysbiotic communities, suggesting distinct host susceptibility. Together, these findings define a population-scale ecological landscape of the subgingival microbiome, reveal divergent trajectories to periodontal dysbiosis, and highlight heterogeneity in the relationship between microbial community structure and clinical disease expression.

microbiology↗
bioRxiv2026

The iron-binding siderophore enterobactin is required for the response of multi-drug resistant Klebsiella pneumoniae to zinc limitation

To persist during infection Klebsiella pneumoniae must overcome nutrient iron and zinc limitation imposed by the host immune system through a process called nutritional immunity. Secreted small molecule siderophores are a major virulence determinant of Klebsiella pneumoniae pathogenesis and are presumed to overcome nutritional immunity by binding iron for bacterial acquisition. In this work, we set out to identify how a multi-drug resistant K. pneumoniae grows in zinc limited environments. Using unbiased transcriptomics, proteomics, and an arrayed transposon screen, we identified that synthesis and uptake of the siderophore enterobactin is required to allow for growth in low zinc conditions. Iron-specific chelators did not replicate this phenotype and addition of supplemental iron through heme in growth media could not complement severe growth defects of enterobactin mutant K. pneumoniae experiencing zinc limitation. Finally, zinc starvation induced enterobactin production independent of the canonical zinc uptake regulator (Zur) transcription factor suggesting an unidentified regulatory mechanism by which Gram-negative pathogens may respond to zinc stress. Together, these studies expand the role of enterobactin beyond iron regulation and highlight a previously unreported link between iron and zinc homeostasis in Klebsiella pneumoniae.

microbiology↗
bioRxiv2026

A microbiota-derived protease links phage susceptibility to host epithelial responses

Bacteriophages are major ecological drivers of gut microbial ecology, yet whether bacterial mechanisms that determine phage susceptibility have consequences for the mammalian host remains poorly understood. Here, we identify dipeptidyl peptidase 11 (Dpp11a), the predominant active serine protease of the prevalent gut commensal Phocaeicola vulgatus, as an unexpected bacterial defence factor. Dpp11a protects against environmental proteases and confers resistance to bacteriophage infection. Metatranscriptomic analyses further reveal increased expression of both dpp11a and P. vulgatus-associated phage transcripts in ulcerative colitis stool samples, indicating that both components of this interaction are transcriptionally active in disease-associated human microbiomes. Using the microfluidic gut-on-a-chip co-culture model HuMiX, we show that the absence of Dpp11 is accompanied by altered epithelial tight-junction remodelling during phage-bacterial infection. Together, our findings reveal that the consequences of bacterial phage defence can extend beyond phage-bacterium interactions to the mammalian epithelium.

microbiology↗