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bioRxiv · 10.1101/2024.11.01.621505

CCR9 signal termination is governed by an arrestin-independent phosphorylation mechanism

Lamme, T. D.·Smit, M. J.·Schafer, C. T.
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Abstract

The chemokine receptor CCR9 coordinates immune cell migration from the thymus to the small intestine along gradients of CCL25. Receptor dysregulation is associated with a variety of inflammatory bowel diseases such as Crohns and ulcerative colitis, while aberrant CCR9 overexpression correlates with tumor metastasis. Despite being an attractive therapeutic target, attempts to clinically antagonize CCR9 have been unsuccessful. This highlights the need for a deeper understanding of its specific regulatory mechanisms and signaling pathways. CCR9 is a G protein-coupled receptor (GPCR) and activates Gi and Gq pathways. Unexpectedly, live-cell BRET assays reveal only limited G protein activation and signaling is rapidly terminated. Truncating the receptor C-terminus significantly enhanced G protein coupling, highlighting the regulatory role of this domain. Signal suppression was not due to canonical arrestin-coordinated desensitization. Rather, removal of GPCR kinase (GRK) phosphorylation led to sustained and robust G protein activation by CCR9. Using site-directed mutagenesis, we identified specific phosphorylation patterns that attenuate G protein coupling. Receptor internalization does not correlate with G protein activation capabilities. Instead, CCR9 phosphorylation appeared to directly destabilize the interaction of G protein heterotrimers with the receptor. This interference could lead to rapid loss of productive coupling and downstream signaling as phosphorylation would effectively render the receptor incapable of G protein coupling. An arrestin-independent, phosphorylation-driven deactivation mechanism could complement arrestin-dependent regulation of other GPCRs and have consequences for therapeutically targeting these receptors.

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Lamme, T. D., Smit, M. J., Schafer, C. T.. 2024-11-03. CCR9 signal termination is governed by an arrestin-independent phosphorylation mechanism. https://doi.org/10.1101/2024.11.01.621505

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