Aberrant iron deposition in the multiple sclerosis spinal cord relates to neurodegeneration

Pisa, M.; Lockhart, A.; Angell, T.; Avery, A.; Waldman, A.; Pansieri, J.; Rodriguez, C.; Duarte, T.; Dhari, Z.; Bailey, M.; Hametner, S.; Drakesmith, H.; Hofer, M.; Limbaeck, C.; DeLuca, G. C.

doi:10.1101/2024.10.25.619794

bioRxiv · 10.1101/2024.10.25.619794

Aberrant iron deposition in the multiple sclerosis spinal cord relates to neurodegeneration

Pisa, M.·Lockhart, A.·Angell, T.·Avery, A.·Waldman, A.·Pansieri, J.·Rodriguez, C.·Duarte, T.·Dhari, Z.·Bailey, M.·Hametner, S.·Drakesmith, H.·Hofer, M.·Limbaeck, C.·DeLuca, G. C.

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Abstract

BackgroundIron accumulates in microglia-macrophages at the edge of multiple sclerosis (MS) lesions in the brain. Iron-rimmed brain lesions strongly predict disability accumulation, supporting iron metabolism is crucial in MS pathology. Little is known about iron distribution in the spinal cord. MethodsAutopsy cervical, thoracic and lumbar spinal cord samples from 9 controls and 46 MS donors of whom a subset (n=36) had mesiofrontal motor cortical tissue available for study, were labelled and systematically assessed for iron (DAB-enhanced Turnbull), myelin (PLP), axons (Palmgren silver), microglia-macrophages (TMEM119, Iba1, CD68), astroglia (GFAP), oligodendroglia (OLIG2), acute axonal injury (B-APP, SMI-32, NPY-1R) and oxidative stress (E06). MS lesional and non-lesional areas were considered. Total non-haem iron was quantified by inductively coupled plasma optical emission spectroscopy (ICP-OES). ResultsIn controls, iron predominantly localised to oligodendrocytes with total non-haem iron relating to total myelin fraction, which markedly differed in MS where iron accumulated in microglia-macrophages, subpial astrocytes, and axons in non-lesional areas. Iron laden microglia-macrophages were over-represented relative to total microglial-macrophages and displayed dysmorphic features. Iron-positive axons showed a disto-proximal gradient (highest at lumbar level) with a predilection for the corticospinal tracts. The extent of iron axon positivity related to smaller spinal cord area, lower total axonal counts, and greater oxidative stress. Iron positivity in each cellular compartment (i.e. subpial astrocytes, microglia-macrophage and axons) related to one-another and total non-haem iron correlated with axonal counts in MS. No iron-rimmed lesions were detected in the spinal cord unlike in cortical grey and subcortical white matter of the same cases where 22% and 80% of iron-rimmed lesions, respectively, were seen. ConclusionsDespite the conspicuous absence of iron-rimmed lesions in the MS spinal cord, we demonstrate widespread aberrant iron distribution in the MS spinal cord that relates to oxidative stress and neurodegeneration independent of demyelination. The distal cord predominant and corticospinal tract specific accumulation of iron in axons mirrors the pattern of length-dependent motoric disability commonly encountered in progressive MS. These findings implicate aberrant iron accumulation as a novel, clinically relevant, feature of MS spinal cord pathology.

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Pisa, M., Lockhart, A., Angell, T., Avery, A., Waldman, A., Pansieri, J., Rodriguez, C., Duarte, T., Dhari, Z., Bailey, M., Hametner, S., Drakesmith, H., Hofer, M., Limbaeck, C., DeLuca, G. C.. 2024-10-27. Aberrant iron deposition in the multiple sclerosis spinal cord relates to neurodegeneration. https://doi.org/10.1101/2024.10.25.619794

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