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bioRxiv · 10.1101/2024.10.24.619789

IL-13/IL-13Rα2 axis promotes proliferation of angiosarcoma cells

Saito, H.·Omori, I.·Iwasawa, O.·Sugimori, A.·Nakajima, H.·Ichimura, R.·Sato, S.·Sumida, H.
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Abstract

Angiosarcoma is a rare and aggressive soft tissue sarcoma with a poor prognosis and limited treatment options. The role of interleukin-13 (IL-13) and its receptors in angiosarcoma pathogenesis has been largely unknown. We detected high IL-13 receptor 2 (IL-13R2) expression in angiosarcoma cell lines and patient samples compared to other cell types and benign vascular tumors. Moreover, histological analysis showed the presence of IL-13 in the angiosarcoma microenvironment. Functional studies using angiosarcoma cell lines, MO-LAS-B cells, revealed the promoting effect of IL-13 on cell proliferation. The effect was inhibited by siRNA-mediated knockdown of IL13RA2 or neutralizing antibodies against IL-13, suggesting the direct impact of IL-13/IL-13R2 axis in the angiosarcoma proliferation. In addition, IL-13 stimulation increased mRNA levels of IL13RA2 and VEGFA, suggesting an underlying positive feedback mechanism, which was attenuated by a STAT6 inhibitor. These findings highlight the importance of the IL-13/IL-13R2 axis in angiosarcoma progression and its potential as a novel therapeutic target for this challenging malignancy.

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Saito, H., Omori, I., Iwasawa, O., Sugimori, A., Nakajima, H., Ichimura, R., Sato, S., Sumida, H.. 2024-10-29. IL-13/IL-13Rα2 axis promotes proliferation of angiosarcoma cells. https://doi.org/10.1101/2024.10.24.619789

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