bioRxiv Science⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2024.10.23.619764

Three-spined sticklebacks recognise familiar individuals by facial recognition

SOGAWA, S.·Inoue, I.·Awata, S.·Ikeya, K.·Kawasaka, K.·Kohda, M.
View original source ↗Export metadata ↓

Abstract

Social vertebrates often recognise familiar individuals by facial recognition, a basal cognitive ability by which animals establish stable sociality, including territoriality. The three-spined stickleback (Gasterosteus aculeatus), a model species for behavioural studies, is territorial; its ability to visually recognise familiar individuals remains unclear. Here, we report that this species has individual-specific facial colour morph features and can recognise familiar individuals by facial recognition. Territorial neighbours of the same sex established a "dear enemy relationship" with each other. These focal fish were exposed to composite photographic models of four combinations of faces and bodies of familiar neighbours and unknown strangers of the same sex. Focal fish of both sexes frequently attacked photographs of strangers (stranger-face/stranger-body) more frequently than familiar neighbours (neighbour-face/neighbour-body). Furthermore, they attacked composite photographs of stranger-face/neighbour-body more frequently (similar to the stranger model) but less frequently attacked photographs of neighbour-face/stranger-body (similar to the neighbour model). These results suggest that the three-spined stickleback distinguishes familiar neighbours from unknown fish exclusively via facial recognition. The aggressiveness of males was independent of the presence of red nuptial colour on photograph-models. Our findings suggest that this fish controls its aggressiveness against opponent conspecifics in the context of social relationships independent of the sign-stimulus.

Explore related subjects

animal behavior and cognitionPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

SOGAWA, S., Inoue, I., Awata, S., Ikeya, K., Kawasaka, K., Kohda, M.. 2024-10-26. Three-spined sticklebacks recognise familiar individuals by facial recognition. https://doi.org/10.1101/2024.10.23.619764

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

A comparison of female competitive traits: Female aggression peaks at nest building but female song spans multiple contexts in a temperate songbird

Female-female competition is increasingly recognized as a key driver of female ornamentation, including birdsong, which often functions in intrasexual competition. However, the specific resources females use elaborate traits to compete for remain unclear. In addition, few studies have simultaneously investigated the use of multiple competitive traits in females, despite growing independent interest in these traits (e.g., female song and aggression). We investigated the competitive contexts of female song, aggression and calling behavior in northern house wrens (Troglodytes aedon) to determine which resources females compete for across the breeding season. We simulated conspecific territorial intrusions using female song at three breeding stages representing different contexts: arrival (mate and territory acquisition), nest building (nest site and breeding status defense), and egg laying (brood defense). We tested whether female song and physical aggression varied as reproductive resources shifted across the breeding cycle. Females were significantly more aggressive during nest building, showing 5.8 times greater odds of a higher-intensity aggressive response during nest building compared to arrival. Female song output was similar across early stages but declined during egg laying, though this was not statistically significant after correction for multiple comparisons and individuals varied substantially in overall singing propensity. Non-song vocalizations varied by call type and breeding stage. Calls associated with aggression occurred most frequently during nest building, consistent with peak physical aggression responses. Together, these results identify nest building as the stage of highest female aggression, consistent with heightened competition over nest cavities and associated breeding status in this cavity-nesting species. In contrast, female song occurred across all stages and appears to function in multiple competitive contexts. This study provides evidence for context and mode-specific female signaling in a temperate songbird and highlights that females strategically use aggression, calls, and song to mediate social conflict across breeding contexts.

animal behavior and cognition↗
bioRxiv2026

Tracking human foragers and their prey reveals adaptive predator-prey dynamics

Hunting for mobile prey is thought to have played a key role in hominin evolution, by providing high-quality nutrition that supported the development of the exceptionally large human brain. However, human-prey dynamics remain poorly understood because studies have not yet tracked human foragers and their prey simultaneously. Here, we employ high resolution tracking of groups of human foragers (ice-fishers) and their prey (fish shoals) to study human-prey dynamics. Our results show that foragers adaptively combined personal and social information in deciding where to forage and for how long, closely matching the prey distribution. Prey responded dynamically to human exploitation, showing increased attraction to fishing activity, alongside decreased biting probability. Furthermore, we found that foragers adaptively relied on memory, preferentially returning to areas with high prey presence, particularly when their current return rate was low. Our results show how human foragers overcome the challenges of extracting invisible, mobile and reactive prey by tightly tuning patch-selection, patch-leaving and patch-return decisions to the distribution and behaviour of their prey.

animal behavior and cognition↗
bioRxiv2026

4-Dimensional Chess: Acoustic Localisation Reveals Nested Spatio-temporal Strategies in an Arboreal Communication Network

1. Adaptive behavioural strategies require animals to simultaneously navigate social and ecological domains across multiple spatial and temporal scales. Although drones and computer vision have recently transformed the study of wild animal societies, many nocturnal species and those occupying structurally complex habitats remain inaccessible to these approaches, limiting our understanding of behaviour in natural settings. 2. We aimed to determine how behavioural strategies are organised across nested spatial and temporal scales within a wild communication network. 3. We used three-dimensional acoustic localisation and source separation to track individual male Hyperolius sp. A, a nocturnal African reed frog, within a natural rainforest chorus and quantify patterns of site fidelity, movement, spatial organisation, and call-timing interactions. 4. Males exhibited significant site fidelity across nights, while chorus spatial structure varied with local caller density. Within nights, individuals followed a stereotyped behavioural sequence, descending from elevated arboreal refugia before settling into lower calling positions near breeding sites. At finer temporal scales, call-timing interactions varied according to both local competitor density and the proximity of neighbouring rivals. 5. These findings demonstrate that behavioural strategies emerge across nested spatial and temporal scales and that long-term spatial positioning, short-term movement decisions, and moment-to-moment signalling interactions are tightly linked within natural communication networks. More broadly, acoustic localisation provides a powerful framework for studying behaviour in species and habitats that remain difficult to observe using conventional approaches.

animal behavior and cognition↗