Methylthio-alkane reductases use nitrogenase metalloclusters for carbon-sulfur bond cleavage

Lago-Maciel, A.; Soares, J. C.; Zarzycki, J.; Buchanan, C. J.; Reif-Trauttmansdorff, T.; Schmidt, F. V.; Lometto, S.; Paczia, N.; Schuller, J. M.; Hansen, D. F.; Heller, G. T.; Prinz, S.; Hochberg, G. K. A.; Pierik, A. J.; Rebelein, J. G.

doi:10.1101/2024.10.19.619033

bioRxiv · 10.1101/2024.10.19.619033

Methylthio-alkane reductases use nitrogenase metalloclusters for carbon-sulfur bond cleavage

Lago-Maciel, A.·Soares, J. C.·Zarzycki, J.·Buchanan, C. J.·Reif-Trauttmansdorff, T.·Schmidt, F. V.·Lometto, S.·Paczia, N.·Schuller, J. M.·Hansen, D. F.·Heller, G. T.·Prinz, S.·Hochberg, G. K. A.·Pierik, A. J.·Rebelein, J. G.

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Abstract

Methylthio-alkane reductases convert methylated sulfur compounds to methanethiol and small hydrocarbons, a process with important environmental and biotechnological implications. These enzymes are classified as nitrogenase-like enzymes, despite lacking the ability to convert dinitrogen to ammonia, raising fundamental questions about the factors controlling their activity and specificity. Here, we present the first molecular structure of the methylthio-alkane reductase, which reveals large metalloclusters, including the P-cluster and the [Fe8S9C]-cluster, previously only found in nitrogenases. Our findings suggest that distinct metallocluster coordination, surroundings, and substrate channels, determine the activity of these related metalloenzymes. This study provides new insights into nitrogen fixation, sulfur-compound reduction, and hydrocarbon production. We also shed light on the evolutionary history of P-cluster and [Fe8S9C]-cluster-containing reductases emerging prior to nitrogenases.

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Lago-Maciel, A., Soares, J. C., Zarzycki, J., Buchanan, C. J., Reif-Trauttmansdorff, T., Schmidt, F. V., Lometto, S., Paczia, N., Schuller, J. M., Hansen, D. F., Heller, G. T., Prinz, S., Hochberg, G. K. A., Pierik, A. J., Rebelein, J. G.. 2024-10-19. Methylthio-alkane reductases use nitrogenase metalloclusters for carbon-sulfur bond cleavage. https://doi.org/10.1101/2024.10.19.619033

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