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bioRxiv · 10.1101/2024.10.15.618603

Meta-analysis: Purity of extracted human blood DNA, E. coli gDNA, and plasmid using magnetic nanoparticle

Tjoa, S. E. E.·Mudasir, M.·Suharyadi, E.·Daryono, B. S.
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Abstract

DNA extraction technology continues to evolve along with the need for a fast and simple process. The DNA extraction with magnetic nanoparticles accommodates these demands by providing a method that is safe, simple, and yet able to give good purity. The purity parameter of the extracted DNA is based on the A260/280 value. This meta-analysis aims to find variables that can affect the purity of extracted DNA using magnetic nanoparticles, based on its extraction and particle synthesis procedure. The information to be analyzed is from DNA extraction from human blood, plasmid extraction, and E. coli gDNA extraction. The research begins with a systematic review to collect appropriate studies and analysis will be carried out using Minitab to find variables that affect the purity of DNA, either positive or negative. Here we show, that no variables have been found significant to the purity of the extracted DNA in both the DNA extraction procedure steps and reagents. For the magnetic nanoparticle synthesis method, for extraction of E. coli gDNA, coating agent TEOS/TiO2; CoZnFe3O4 magnetic nanoparticles, and the interaction between CoZnFe3O4 and TEOS are found to be variables that influence the purity of the extracted DNA based on A260/280. Hopefully, this information can be used as a development idea, either for the magnetic nanoparticle synthesis procedure or the selection of reagents and DNA extraction procedures. In addition, the results of this analysis can also be used for consideration in an in-house magnetic nanoparticle-based DNA extraction assembly.

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Tjoa, S. E. E., Mudasir, M., Suharyadi, E., Daryono, B. S.. 2024-10-16. Meta-analysis: Purity of extracted human blood DNA, E. coli gDNA, and plasmid using magnetic nanoparticle. https://doi.org/10.1101/2024.10.15.618603

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